藤梨根药材HPLC指纹图谱及质量研究

目的:建立藤梨根药材的HPLC-UV特征指纹图谱,为其品质评价提供参考.方法:以乙腈-0.25％甲酸水为流动相梯度洗脱,测定波长280 nm,指纹图谱相似度评价软件确定共有峰后,建立藤梨根的标准指纹图谱并计算了每批药材的相似度,利用聚类分析方法对指纹图谱数据进行分析.结果:建立了含有15个共有指纹峰的藤梨根HPLC-UV特征指纹图谱,并标定熊果酸、油酸、阿魏酸、槲皮素等7种共有成分.结论:该方法快速、准确,可用于评价藤梨根药材质量.

Study on HPLC Fingerprint and Quality Control of Radix Actinidiae

Objective: To establish a sensitive and specific HPLC method and a standard fingerprint for quality control of Radix Actinidiae. Method: The HPLC characteristic chromatographic profiles of 11 samples from different sources were eluted with the mobile phases of water containing 0.25% formic acid and acetonitrile in gradient mode. The detection wavelength was set at 280 nm. By the similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (Version 2004 A),mean chromatogram was generated as the representative standard fingerprint and the similarity of each chromatogram against the standard chromatogram was also calculated. Peaks were applied to grade by hierarchical cluster analysis. Result: Fifteen main marker peaks were selected in the standard fingerprint and 7 peaks of them were identified. Conclusion: The quality control of crude drug can be evaluated by comparing with the standard fingerprint. The method was rapid, accurate, and can be applied for quality assessment of Radix Actinidiae.