| C-Met抑制剂对人晶状体上皮细胞增殖的抑制作用 |
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| 引用本文： | 安小玲,王维明,韩清,陶津华,张劲松.C-Met抑制剂对人晶状体上皮细胞增殖的抑制作用[J].国际眼科杂志,2006,6(4):743-744. |
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| 作者姓名： | 安小玲 王维明 韩清 陶津华 张劲松 |
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| 作者单位： | 110005,中国辽宁省沈阳市,中国医科大学附属第四医院眼科;152400,中国黑龙江省庆安县,黑龙江省庆安县人民医院眼科;150001,中国黑龙江省哈尔滨市,哈尔滨医科大学附属第四医院眼科 |
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| 摘 要： | 目的:探讨 c-M et抑制剂 K252a 对人晶状体上皮细胞增殖的抑制作用。方法 : 取原代培养人晶状体上皮细胞加入 H G F、H G F K252a,以 D M EM 为对照,应用四唑盐法(M TT法)观察 K252a 对晶状体上皮细胞生长的抑制作用,蛋白质免疫印迹(W estern blot)方法检测 K252a 对 Bcl-2,C aspase-3 蛋白表达的影响。结果:H G F (50nm ol/L) K252a(30nm ol/L)组与对照组光密度值无显著差异 (P>0.05),Bcl-2 和 C aspase-3 的表达水平变化都不明显。结论:c-M et抑制剂 K252a 对人晶状体上皮细胞增殖有抑制作用。
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| 关 键 词： | 晶状体上皮细胞 c-Met K252a Bcl-2 Caspase-3 |
| 收稿时间： | 06 6 2006 12:00AM |
| 修稿时间： | 07 30 2006 12:00AM |
Inhibition effect of c-Met inhibitor on proliferation of lens epithelial cells |
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| Xiao-Ling An,Wei-Ming Wang,Qing Han,Jin-Hua Tao,Jin-Song Zhang.Inhibition effect of c-Met inhibitor on proliferation of lens epithelial cells[J].International Journal of Ophthalmology,2006,6(4):743-744. |
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| Authors: | Xiao-Ling An Wei-Ming Wang Qing Han Jin-Hua Tao Jin-Song Zhang |
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| Abstract: | AIM: To observe the inhibition of c-Met inhibitor on proliferation of lens epithelial cells (LECs).METHODS: Human's LECs were cultured and hepatocyte growth factor (HGF) and K252a were added to second passage of cells supplied with Dulbecco's modified eagle's medium (DMEM). MTT assay was used to examine the proliferation of LECs, and Western-blot was used to detect the expression change of Bcl-2 and Caspase-3.RESULTS: The photodensity (A) of HGF (50nmol/L) + K252a (30nmol/L) was not significantly different from that of DMEM control (P＞0.05). The expression of Bcl-2 and Caspase-3 were not significantly different from that in the control group.CONCLUSION: K252a, the inhibitor of c-Met, can effectively inhibit the proliferation of LECs. |
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| Keywords: | lens epithelial cells c-Met K252a Bcl-2 Caspase-3 |
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