三氧化二砷对C6胶质瘤细胞增殖和凋亡的影响

目的 探讨三氧化二砷(As2O3)对体外培养的C6胶质瘤细胞增殖和凋亡的影响.方法 以浓度为1、3、5μmol/LAs2O3分别作用于体外培养的C6胶质瘤细胞,采用甲基噻唑蓝比色法(MTT)检测细胞活性,流式细胞仪检测细胞周期和细胞凋亡,并用倒置显微镜及透射电子显微镜观察细胞形态学和超微结构的变化.结果 经1、3、5μmol/L As2O3作用后,MTT检测C6胶质瘤细胞活性受到抑制,且呈剂量时间依赖性;流式细胞仪检测凋亡细胞数目随着As2O3浓度的增加呈现逐渐递增的趋势;且上述药物干预者与未用药物干预者比较差异均有统计学意义(P＜0.05);透射电子显微镜检测药物干预者C6胶质瘤细胞较未用药物干预者体积明显缩小,可见少量空泡,细胞核不规则,核内染色质凝聚、边集,形成凋亡细胞的形态学改变等现象.结论 As2O3可抑制C6胶质瘤细胞增殖,诱导C6胶质瘤细胞凋亡.

Abstract：

Objective To investigate the effects of arsenic trioxide(As2O3) on the cell proliferation and apoptosis of C6 glioma cells in vitro.Methods The C6 glioma cells were treated by 1,3,5 μ mol/L of As2O3 with different duration and observed under the microscope and electromicroscope.The viability of C6 glioma cells was examined by methyl thiazolyl tetrazolium (MTT) assay,and cell cycle and apoptosis were examined by flow cytometry.Results After treatment of 1,3,5 μ mol/L As2O3,C6 glioma cells were inhibited obviously with a dose- and time-dependent manner (P ＜0.05) by MTT.During flow cytometry,more increasing apoptotic cells were found in different concentration As2O3.Characteristic morphological changes were observed in As2O3 intervention by transmission election microscopy including cell shrinkage,physaliphore,nuclear condensation and apoptosis and so on.Conclusion As2O3 can inhibit the cell proliferation and induce the apoptosis of C6 glioma cells.

Effects of arsenic trioxide on cell proliferation and apoptosis in C6 glioma cell

Objective To investigate the effects of arsenic trioxide(As2O3) on the cell proliferation and apoptosis of C6 glioma cells in vitro.Methods The C6 glioma cells were treated by 1,3,5 μ mol/L of As2O3 with different duration and observed under the microscope and electromicroscope.The viability of C6 glioma cells was examined by methyl thiazolyl tetrazolium (MTT) assay,and cell cycle and apoptosis were examined by flow cytometry.Results After treatment of 1,3,5 μ mol/L As2O3,C6 glioma cells were inhibited obviously with a dose- and time-dependent manner (P ＜0.05) by MTT.During flow cytometry,more increasing apoptotic cells were found in different concentration As2O3.Characteristic morphological changes were observed in As2O3 intervention by transmission election microscopy including cell shrinkage,physaliphore,nuclear condensation and apoptosis and so on.Conclusion As2O3 can inhibit the cell proliferation and induce the apoptosis of C6 glioma cells.