| 葡萄糖抑制THP-1巨噬细胞载脂蛋白E的表达 |
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| 引用本文： | 刘晓燕,严士敏,赵秀丽,郁红,陈凤玲.葡萄糖抑制THP-1巨噬细胞载脂蛋白E的表达[J].蚌埠医学院学报,2009,34(11):953-955. |
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| 作者姓名： | 刘晓燕 严士敏 赵秀丽 郁红 陈凤玲 |
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| 作者单位： | 上海交通大学医学院附属第三人民医院内分泌科, 201900 |
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| 基金项目： | 国家自然基金资助项目，上海市科委自然科学基金资助项目 |
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| 摘 要： | 目的:观察葡萄糖对人单核细胞性白血病细胞株(human monocytic leukemia cell line,THP-1)巨噬细胞的载脂蛋白E(Apo E)表达的影响。方法:用佛波脂诱导THP-1细胞使其分化成巨噬细胞。再用含不同浓度葡萄糖的RPMI 1640培养基培养巨噬细胞72 h后,RT-PCR和Western Blot分别检测巨噬细胞Apo E的mRNA和蛋白的表达,同时用MTT法检测各组细胞活力。结果:葡萄糖呈剂量依赖性(20~30 mmol/L),明显抑制THP-1巨噬细胞Apo E的表达。结论:葡萄糖抑制THP-1巨噬细胞Apo E的表达,其可能是导致动脉粥样硬化的机制之一。
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| 关 键 词： | 动脉粥样硬化 巨噬细胞 血糖过多 载脂蛋白E |
| 收稿时间： | 2009-03-24 |
Glucose decreases apolipoprotein E expression in THP-1 macrophages |
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| LIU Xiao-yan,YAN Shi-min,ZHAO Xiu-li,YU Hong,CHEN Feng-ling.Glucose decreases apolipoprotein E expression in THP-1 macrophages[J].Journal of Bengbu Medical College,2009,34(11):953-955. |
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| Authors: | LIU Xiao-yan YAN Shi-min ZHAO Xiu-li YU Hong CHEN Feng-ling |
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| Institution: | Department of Endocrinology, No.3 People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201900, China |
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| Abstract: | Objective:To observe the influence of glucose on the expression of Apolipoprotein E(Apo E) in human monocytic leukemia cell line(THP-1) macrophages.Methods:THP-1 cells were induced to macrophages with phorbol,and then treated with RPMI 1640 culture medium containing diferent concentrations of glucose for 72 hours.Apo E mRNA and protein level were analyzed by RT-PCR and Western Blot respectively,meanwhile the viability of macrophages was assayed with MTT method.Results:Glucose significantly decreased Apo E e... |
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| Keywords: | atherosclerosis macrophage hyperglycemia apolipoprotein E |
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