环氧合酶-2在c9,t11-共轭亚油酸抑制肿瘤细胞转移中的作用

目的 研究c9,t11-共轭亚油酸（c9,t11-CLA）抑制人胃腺癌细胞（SGC-7901）转移作用与亚油酸代谢途径中限速酶环氧合酶-2（COX-2）的关系。方法采用体外细胞培养方法,用COX-2的选择性抑制剂NS．398抑制SGC-7901细胞中COX-2的活性,再加入200、100、50和25μmol／L浓度的c9,t11-CLA,利用重组基底膜侵袭实验、黏附实验、趋向运动实验检测c9,t11-CLA抑制肿瘤细胞转移的作用与COX-2的关系。结果与NS-398组比较,NS-398＋100Ixmol／Lc9,t11-CLA和NS-398＋200μmol／L c9,t11-CLA对SGC-7901细胞的侵袭有明显的抑制作用,穿过膜的细胞数分别为48．7±1．5（F=14．309,P=0．000）和43．7±4．0（F=19．005,P=0．000）;NS-398＋200μmol／L c9,t11-CLA组能显著降低SGC-7901细胞对基质成分层粘连蛋白、纤维粘连蛋白和基质胶的黏附作用,所测吸光度值（A值）分别为0．052±0．011,0．058±0．008（F=3．063,P=0．021）和0．042±0．004（F=6．692,P=0．001;F=11．999,P=0．000）;NS-398＋200ixmol／L c9,t11-CLA对SGC-7901细胞的趋向运动能力无明显的抑制作用（F=1．380,P=0．276）,其中NS-398＋2001xmol／L c9,t11-CLA组细胞数为26．6±3．4。结论 c9,t11-CLA具有抑制SGC-7901细胞体外侵袭能力、黏附能力和趋向运动能力,这种抑制作用可能与COX-2途径有关。

Study on inhibitory effects of c9, t11-conjugated linoleic acid on migration of human gastric carcinoma cell line via cyclooxygenase-2 pathway

OBJECTIVE: To study the inhibitory effects of c9, t11-conjugated linoleic acid (c9, t11-CLA) on migration of human gastric carcinoma cell line (SGC-7901) via cyclooxygenase-2 (COX-2) pathway. METHODS: After inhibiting COX-2 activity by 100 micromol/L COX-2 inhibitor NS-398 in SGC-7901 cell, we treated SGC-7901 cells with c9, t11-CLA at a concentration of 200,100, 50, 25 micromol/L for 24 h, respectively. Using reconstituted basement membrane invasion, adhesion, chemotaxis assays, we detected the effect of c9, t11-CLA and COX-2 on the cell migration. RESULTS: Compared to NS-398 group, 200, 100 micromol/L c9, t11-CLA significantly suppressed SGC-7901 cells invading into the reconstituted basement membrane (F = 14.309, P = 0.000; F = 19.005, P = 0.000). 200 micromol/L c9, t11-CLA significantly inhibited SGC-7901 cells adhering to laminin, fibronectin and Matrigel (F = 3.063, P = 0.021; F = 6.692, P = 0.001; F = 11.999, P = 0.000). The chemotaxis of SGC-7901 cells and inhibitory frequency were significantly decreased in the 200 micromol/L c9, t11-CLA group (F = 1.380, P = 0.276). CONCLUSION: c9, t11-CLA inhibits invasion, adhesion and chemotaxis of SGC-7901 cells, and the COX-2 plays an important role in the process. Key words]