大豆异黄酮对骨质疏松症动物模型骨代谢及形态学的影响

目的 研究大豆异黄酮（SI）对骨质疏松症动物模型骨代谢及形态学的影响。方法70只雌性SD大鼠按血清总胆固醇（TC）水平用随机数字表法分为7组：高脂组、雌激素组、SI低、中、高剂量组,假手术与正常对照组,前5组摘除双侧卵巢。除正常对照组外,其余各组给予高脂饲料饲养。每周称重1次,灌胃给予受试物,干预周期为12周,分别于去卵巢、给药4、8周、处死前抽取尾静脉血,实验结束后取内脏及骨骼标本,分别测定血清碱性磷酸酶（AKP）活性及骨密度等指标,共63只大鼠完成实验。结果 去卵巢后雌激素与SI干预可恢复AKP活性,高剂量SI干预对维持大鼠骨密度的作用与雌激素相似并能缓解因去卵巢造成骨丢失的显微镜像、维护心肌细胞肌微丝与线粒体超微结构的完整性,全视野下以雌激素组、高剂量干预组与正常组结构最为接近,高脂组显微结构损坏明显。结论 SI对去卵巢大鼠的骨代谢及形态学存在一定影响,高剂量干预可使去卵巢大鼠血清AKP酶活性恢复、逆转因去势造成的骨密度下降、维持血清钙、磷水平,低剂量效果不显著。

Effects of soybean isoflavone on born metabolism and morphology in animal model of osteoporosis rats

OBJECTIVE: To study the effects of soybean isoflavone (SI) on born metabolism and morphology in animal model of osteoporosis rats. METHODS: All 70 female Sprague-Dawley (SD) rats were randomly divided into 7 groups according to the levels of total cholesterol (TC) in serum: hyper-lipoid group, estrogen group, low-dose SI group, middle-dose SI group, high-dose SI group, sham group and normal control groups. Bilateral ovaries were extirpated except sham and normal control groups. Except the rats in normal control group, the other rats were fed with high fat diet. Body weight was weighted ad unam vicem per week. The estrogen, different dose of SI or deionized water were fed with intragastric administration for 12 weeks. Vena caudalis serum were collected after being ovariectomized, administered for 4 w, 8 w and killed. Serum alkaline phosphatase (AKP) activity and bone density were measured etc. RESULTS: To interfere of estrogen and SI might recover AKP enzyme activity after its being ovariectomized. There almost sowed no differences between high dose SI intervention and estrogen on bone density and microstructure. Bone loss due to being ovariectomized was relieved after SI intervention. SI might protect cardiocyte myofilament and mitochondrial ultramicrostructure. There was mirror image in estrogen, high dose SI group resembling the normal control group, and there was obvious damage in hyper-lipoids group. CONCLUSION: There should be effects of high dose SI on bone metabolism and morphology in animal model of osteoporosis rats. Serum AKP enzyme activity and bone density should have significantly recovered, the serum level of calcium and phosphorus were maintained after high dose intervented but no significant effects for low dose of SI.