芪丹颗粒剂预防和延缓鼠矽肺纤维化的作用

目的 探讨芪丹颗粒剂预防性治疗矽肺纤维化的作用。方法 40只Wistar大鼠分为：正常对照组、模型组、芪丹组、汉甲组4个组。除正常对照组外,其余各组采用气管注射染尘（每只鼠50mg）方法建立动物模型,芪丹组和汉甲组分别从造模后第2天灌注芪丹颗粒剂（3125mg／kg）和汉防己甲素（22mr／kg）;5个月后处死,进行肺泡灌洗,取支气管肺泡灌洗液（ bronchoalveolar lavage fluid, BALF）、肺脏、肾脏标本。称取大鼠体重、鼠肺湿重,计算大鼠肺系数;用碱水解法测定羟脯氨酸含量;用酶联免疫吸附测定法（ELISA）测定肺泡灌洗液中转化生长因子β1（TGF-131）的表达;同时对肺组织作HE染色、VG染色和网状纤维染色（gomorri）,普通光镜下观察各组矽结节的细胞构成,胶原纤维及网状纤维改变;同时取肾组织作HE染色,普通光镜下观察肾脏病理改变。结果 芪丹组、汉甲组的肺系数、羟脯氨酸含量、BALF中TGF-131的表达均低于模型组,差异有统计学意义。模型组主要以Ⅲ～Ⅳ级矽结节为主,胶原纤维粗大、排列致密,网织纤维稀疏,芪丹颗粒及汉防己甲素预防治疗组主要以Ⅱ级矽结节为主,胶原纤维细小、排列疏松,网织纤维密集;汉甲组大鼠出现肾损伤,其余各组大鼠肾组织正常。结论 芪丹颗粒剂有预防和延缓矽肺纤维化的作用,安全无肾毒性。

The anti-fibrotic effects of Qidan granule in experimental silicosis

OBJECTIVE: To investigate the anti-fibrotic effects of Qidan granule in rats. METHODS: The rats were randomly divided into six experimental groups: normal group, model group, Qidan group, Tetrandrine group. All rats except normal group were treated with silicon dioxide (50 mg/rat) by intratracheal instillation to induce silicosis. Qidan group and Tetrandrine group were treated with Qidan granule (3125 mg/kg) or treated with Tetrandrine (22 mg/kg) respectively. All the rats were sacrified after 5 months. Calculate Lung/body coefficient by weighting the lung wet weight and the body weight of rats. Content of Hydroxyproline was measured by alkaline hydrolysis. The gene expression of transforming growth factor-beta1 was examined by using enzyme-linked immunosorbent assay (ELISA). Paraffinembedded lung sections with HE staining, VG staining and Gomorri staining were observed under light microscope. RESULTS: In Qidan group and Tetrandrine group, Lung/body coefficient and content of Hydroxyproline and expression of transforming growth factor-beta1 were lower as compared with model group (P < 0.05). Model group mainly showed III approximately IV grade silicotic nodule, which contained thick collagen and sparse reticulum fibe; Qidan group and Tetrandrine group appeared with II grade silicotic nodule, which contained tiny collagen and intensive reticulum fibe. Tetrandrine group showed injury of kidney, and others were normal. CONCLUSION: Qidan granule extract should prevent and from inhibit the remarkably silicotic fibrosis in rats.