| Abstract: | objective: To determine the DNA damage, neuronal and glial cells apoptosis and expression of P53 protein in the rat spinal cord after compression injury. Methods: Terminal deoxynucleotidyl transferase . mediated dUPT nick end labeling (TUNEL), DNA gel electrophoresis and immunohistochemistry techniques were used to detect DNA fragmentation in the injured rat spinal cord. Results: The apoptosic cells and P53 protein presented at 4 h after spinal cord injury with a maximum presence at 24 h in the injuried T8.9 DNA fragmentation presented typical ladder pattern on agarose gel at 24 h. Conclusion: There are lots of neuronal and glial cells apoptosis with DNA damage after SCl. The P53 protein may play an important role in induc tion of neuronal and glial cells to apoptosis. |
