新型免疫抑制剂J2对角膜移植小鼠淋巴细胞中白细胞介素10及干扰素γ分泌的影响

背景：小分子化合物J2是以CD4为靶点的新型免疫抑制剂,课题组以往的实验已经验证了J2对正常小鼠及角膜移植后小鼠脾细胞的影响。目的：探讨小分子化合物J2对异基因角膜小鼠的淋巴细胞中白细胞介素10及干扰素γ分泌的影响。方法：BalB/c（H2d）小鼠随机数字表法分为4个组,安慰剂组、环孢素A组和J2组接受C57BL/6-BALB/c同种异体角膜移植后给予相应药物干预,空白对照组不接受角膜移植,观察各组角膜移植片存活时间。取各组大鼠脾细胞给予刀豆蛋白Ⅵ型刺激细胞增生,采用ELISA法测定细胞上清中白细胞介素10及干扰素γ水平,比较各组细胞增生指数及细胞因子含量。结果与结论：J2可能通过抑制CD4＋T淋巴细胞而抑制角膜排斥反应的发生,延长角膜植片存活时间;J2能够明显抑制ConA刺激角膜移植后小鼠脾细胞的增生及Th1细胞因子的产生。这些效应与环孢素A的效果相似。

Effects of a new immunodepressant J2 on lymphocytic secretion of interleukin 10 and interferon gamma in mice after corneal allograft

BACKGROUND：J2 is a new immunodepressant targeting CD4, and previous studies have verified the effect of J2 on spleen cells of normal mice or mice undergoing corneal transplantation. OBJECTIVE：To investigate the effects of J2 on the lymphocytic secretion of interferon gamma （IFN-γ） and interleukin 10 （IL-10） of mice with allogeneic corneal transplantation. METHODS：Corneal transplantation models were created in the right eyes of 36 clean BalB/c（H2d） mice （receiptors） and 17 clean C57BL/6（H2b） mice （donors）. The models were randomly divided into four groups according to the different intervene methods. Corneal opacification and neovascularization were scored. The proliferation of spleen cells of mice was detected by ATPase method, and secretion of IL-10 and IFN- γ in cytosupernatant before and after ConA stimulated were assessed by ELISA. RESULTS AND CONCLUSION：J2 arrest the occurrence of corneal rejection by inhibiting the activation of CD4＋ T cells and suppress the proliferation and ConA-stimulated Th1 cytokine production of spleen cells.