间歇缺氧及睡眠剥夺大鼠血管内皮细胞相关指标的变化

背景：血管内皮功能损坏是睡眠呼吸暂停的病理基础。目的：观察间歇缺氧、睡眠剥夺对SD大鼠有创动脉收缩压及血浆一氧化氮、内皮素、降钙素基因相关肽水平的影响。方法：将3月龄雄性SD大鼠16只随机等分为2组,模型组大鼠每天置入睡眠剥夺合并间歇性缺氧条件10h（22：00-08：00）,单纯睡眠剥夺条件12h（08：00-20：00）,剩余时间置大鼠笼饲养。对照组无睡眠剥夺、无缺氧条件饲养。结果与结论：造模8周后,与对照组比较,模型组大鼠有创动脉压明显升高（P〈0.01）,血浆一氧化氮、降钙素基因相关肽水平显著降低（P〈0.01）,血浆内皮素水平显著升高（P〈0.01）。说明间歇性缺氧、睡眠剥夺可以引起SD大鼠血压增高,血管内皮功能受损。

Changes in vascular endothelial cells-related indices in rats with intermittent hypoxia and sleep deprivation

BACKGROUND：Sleep apnea syndrome has been considered be a significant independent factor that caused outbreaks of hypertension and coronary heart disease. Vascular endothelial dysfunction may be one of the most important mechanisms for the manifestation of these diseases. OBJECTIVE：To investigate the effects of intermittent hypoxia and sleep deprivation on invasive arterial blood pressure, plasma nitric oxide, endothelin and calcitonin gene related protein levels in rats. METHODS：Sixteen 3-month-old male Sprague-Dawley rats were randomly and evenly divided into two groups：the control group and the intermittent hypoxia and sleep deprivation group. Rats in the intermittent hypoxia and sleep deprivation group were placed in an environment of intermittent hypoxia and sleep deprivation for 10 hours （22：00-08：00） and in an environment of simple sleep deprivation for 12 hours （08：00-20：00）. During the remaining time, the rats were raised in cages. control group rats were raised without sleep deprivation and intermittent hypoxia. RESULTS AND CONCLUSION： After 8 weeks of model establishment, compared with the control group, invasive arterial blood pressure was significantly higher （P 0.01）, plasma nitric oxide and calcitonin gene related protein levels were significantly decreased （P 0.01）, and plasma endothelin level was significantly increased （P 0.01） in the intermittent hypoxia and sleep deprivation group. These results showed that intermittent hypoxia exposure and sleep deprivation can cause increased blood pressure and vascular endothelial dysfunction in Sprague-Dawley rats.