血清体积分数对人细胞因子诱导杀伤细胞增殖的影响

背景：细胞因子诱导的杀伤细胞的培养多采用体积分数10%的血清,但关于血清体积分数对其生长特性的影响少有报道。目的：观察细胞因子诱导的杀伤细胞在不同血清体积分数培养液中的增殖情况。方法：分别配制血清体积分数为10%,20%和30%＋重组人白细胞介素2等细胞因子的完全培养液培养人细胞因子诱导的杀伤细胞,使用流式细胞仪分析血清体积分数对细胞周期的影响,通过MTT法检测细胞因子诱导的杀伤细胞在不同血清体积分数培养液中的增殖,并观察细胞因子诱导的杀伤细胞的生长状态。结果与结论：细胞因子诱导的杀伤细胞在体积分数10%血清培养液中增殖速率和增殖指数均最低,在体积分数30%血清培养液中最高,3组细胞的不同时间点的增殖速率差异均有显著性意义（P〈0.05或P〈0.01）。结果证实,体外培养的细胞因子诱导的杀伤细胞的增殖具有血清体积分数依赖性,高血清体积分数培养液的促细胞因子诱导的杀伤细胞增殖效应明显。

Effects of serum concentration on the proliferation of human cytokine induced killer cells

BACKGROUND：Cytokine induced killer（CIK） cells are cultured mostly in 10% serum,but there have been few studies describing the effects of serum concentration on growth characteristics of CIK cells.OBJECTIVE：To investigate the proliferation of CIK cells in different concentrations of serum.METHODS：CIK cells were cultured with 10%,20% and 30% fetal bovine serum supplemented with recombinant human interleukin（IL-2）.The effects of serum concentration on cell cycle were analyzed using flow cytometry.The proliferation of CIK in different concentrations of serum was determined by MTT assay and the growth characteristics of CIK cells were observed.RESULTS AND CONCLUSION：The proliferation rate and proliferation index of CIK cells were the lowest in 10% serum and highest in 30% serum.There was statistical significance in proliferation rate among three groups（P 0.05 or P 0.01）.The proliferation of CIK cells cultured are serum concentration-dependent in vitro.