定制型聚羟基乙酸/聚乳酸支架与犬骨髓基质细胞的体外复合培养

背景：聚羟基乙酸、聚乳酸均属于脂肪族聚酯,是一种具有一定机械强度和良好成型性能的生物可降解材料,在体内无毒,不聚积,且有良好的生物相容性。目的：应用CAD、CAM、快速成型和激光扫描技术等组成的数字医学系统制作聚羟基乙酸/聚乳酸三维仿真的下颌支髁突形态模型,并检测其细胞生物相容性。方法：通过CT扫描获得犬头颅骨影像信息,以CAD和CAM实现下颌骨髁突形态的三维重建影像,快速成型技术获得下颌骨髁突的树脂阳模。阴阳模转换获得相应石膏阴模,聚羟基乙酸/聚乳酸在阴模内成型。抽取犬髂骨骨髓获得骨髓基质细胞,与定制型聚羟基乙酸/聚乳酸支架在体外复合培养,检测支架材料的生物相容性。结果与结论：定制型聚羟基乙酸/聚乳酸支架和影像原型比较,当测试点误差小于1.0mm时,复合率大于95%。通过CAD、CAM、快速成型技术、预压成型技术和激光扫描技术等组成的数字医学系统可实现颅颌面下颌骨髁突形态结构聚羟基乙酸/聚乳酸生物材料的三维仿真。体外复合培养结果表明,定制型聚羟基乙酸/聚乳酸支架和骨髓基质细胞具有良好的生物相容性。

Custom-made polyglycolic acid/polylactic acid scaffolds cocultured with bone marrow stroma cells of canine in vitro

BACKGROUND：Polyglycolic acid（PGA） and polylactic acid（PLA） are belonging to the aliphatic polyester,which is a certain mechanical strength and good shaping properties of biodegradable material,innocuity in vivo,without accumulation,and has a good biocompatibility. OBJECTIVE：To produce PGA/PLA three-dimensional simulation of the mandibular condylar shape model by using of CAD,CAM,rapid prototyping and laser scanning technology and other components of digital medical systems,and to detect its cell biocompatibility. METHODS：Parietal bone image information of canine was obtained by CT scan,condyle of mandible morphology three-dimensional reconstruction images were performed by CAD and CAM,resin male of condyle of mandible was obtained by rapid prototyping technology. Yin-yang male was transformed to obtain the corresponding plaster cavity block;PGA/PLA was formed in the cavity block. Canine iliac bone marrow was abstracted to achieve bone marrow stroma cells（BMSCs） . BMSCs cocultured with custom-made PGA/PLA scaffolds in vitro to detect the biocompatibility of scaffolds. RESULTS AND CONCLUSION：Compared with image prototype,when the test point error is less than 1.0 mm,the compound rate is more than 95% in PGA/PLA scaffolds. According to CAD,CAM,rapid prototyping technology,pre-molding technology,laser scanning technology,and other components of digital medical systems can achieve three-dimensional stimulation of craniofacial mandibular condyle morphosis PGA/PLA biomaterial. The results of cocultured in vitro showed that custom-made PGA/PLA and BMSCs have a good biocompatibility.