胚胎骨髓来源间充质干细胞对人Th17细胞的免疫调节

背景：众多研究表明间充质干细胞能发挥免疫调节功能,抑制T细胞增殖。目的：观察胚胎骨髓来源间充质干细胞对人Th17细胞的调节作用。方法：将人胚胎骨髓间充质干细胞与正常人外周血单个核细胞或CD4＋T细胞以1∶10比例共培养4d,以单个核细胞或CD4＋T细胞单独培养为对照。应用实时定量PCR检测细胞白细胞介素17mRNA表达,酶联免疫吸附试验检测细胞上清中白细胞介素17蛋白水平,流式细胞术检测Th17细胞数量。结果与结论：胚胎骨髓来源间充质干细胞与单个核细胞共培养组白细胞介素17mRNA表达水平明显高于单个核细胞组（P〈0.01）。与此一致的是,胚胎骨髓来源间充质干细胞与单个核细胞或CD4＋T细胞共培养组细胞上清中白细胞介素17蛋白水平明显高于单个核细胞组、CD4＋T细胞组（P〈0.05,P〈0.01）。胚胎骨髓来源间充质干细胞与CD4＋T细胞共培养组Th17细胞数量明显高于CD4＋T细胞组（P〈0.01）,但胚胎骨髓来源间充质干细胞本身并不表达白细胞介素17。表明胚胎骨髓来源间充质干细胞可促进人Th17细胞增殖。

Immunoregulatory function of fetal bone marrow-derived mesenchymal stem cells on human Th17 cells

BACKGROUND： Many studies have demonstrated mesenchymal stem cell （MSC） can regulate the immune function and inhibit proliferation of T cell. OBJECTIVE： To investigate the regulatory function of fetal bone marrow-derived mesenchymal stem cells （FBM-MSCs） on human Th17 cells. METHODS： FBM-MSCs were cocultured with human peripheral blood mononuclear cells （PBMCs） or CD4＋ T cells at 1：10 ratio from healthy donors for 4 days. Single culture of mononuclear cells or CD4＋ T cells were as controls. The expression of interleukin-17 （IL-17） mRNA was detected by real-time PCR, IL-17 protein was assayed by enzyme-labeled immunosorbent assay （ELISA）, and quantity of Th17 cells was observed by flow cytometry. RESULTS AND CONCLUSION： The level of IL-17mRNA in FBM-MSC cocultured with PBMC （FBM-MSC/PBMC） group was significantly higher than that in PBMC cultured alone. Meanwhile, IL-17 expression in supernatant in FBM-MSC/PBMC/ CD4＋ was significantly higher than that in PBMC and CD4＋ T cells groups （P 0.05, P 0.01）. The number of FBM-MSCs/CD4＋ T cells group was significantly more than CD4＋ T cells group （P 0.01）. However, FBM-MSCs did not express IL-17 protein. FBM-MSCs can promote the proliferation of Th17 cells.