许旺细胞联合C5a受体拮抗剂移植脊髓损伤大鼠的电生理及后肢功能变化

背景：C5a通过增强脊髓损伤后早期炎症反应参与了脊髓损伤后的继发性损伤,C5a受体拮抗剂能有效阻断这一过程。目的：观察许旺细胞移植联合C5a受体拮抗剂对脊髓损伤大鼠神经功能恢复的影响。方法：Wistar大鼠80只建立脊髓损伤动物模型后随机分成4组。①空白对照组尾静脉注射培养液组＋腹腔注射生理盐水。②细胞移植组尾静脉注射许旺细胞。③C5a受体拮抗剂组腹腔注入C5a受体拮抗剂。④联合组尾静脉注射许旺细胞,同时腹腔注入C5a受体拮抗剂。结果与结论：下肢运动功能评价联合移植组优于细胞移植组和C5a受体拮抗剂组,细胞移植组和C5a受体拮抗剂组优于对照组。细胞移植组和联合组有SRY基因表达。HRP阳性神经纤维数：联合组〉胞移植组与C5a受体拮抗剂组〉空白对照组,且各组之间差异有显著性意义（P〈0.01）。联合组大鼠体感诱发电位及运动诱发电位的潜伏期、波幅明显优于其他3组（P〈0.05或P〈0.01）。提示许旺细胞移植和C5a受体拮抗剂联合应用可促进脊髓损伤大鼠神经突触的再生,改善其肢体运动功能和电生理功能。

Effects of complement 5a receptor antagonists combined with Schwann cells transplantation on recovery of electrophysiological and neurological function in rats with spinal cord injury

BACKGROUND：Complement 5a（C5a） by enhancing the early inflammatory response after spinal cord injury participates in the spinal cord secondary injury.C5a receptor antagonists can effectively block this process.OBJECTIVE：To investigate the effect of Schwann cells transplantation and C5a receptor antagonists on repair of rat spinal cord injury.METHODS：Eighty healthy female Wistar rats were randomly divided into 4 groups：control group,cell transplantation group,C5a receptor antagonist group and combination group,20 rats in each group.RESULTS AND CONCLUSION：The outcome of combination group was superior to the cell transplantation group and the C5a receptor antagonist group,and the cell transplantation group and the C5a receptor antagonist group was superior to the control group（P〈0.05）.Cell transplantation group compared with the C5a receptor antagonist was no significant difference（P〈0.05）.The SRY expression in cell transplantation group and combined group,in contrast,no SRY gene were detected in control group and the C5a receptor antagonist group.The number of HRP-positive nerve fibers：the combination group cell transplantation group and C5a receptor antagonist group control group and the difference between the groups was significant（P〈0.01）.Somatosensory evoked potential,motor evoked potential latency,and amplitude were significantly better in the combination group than the other three groups（P〈0.05 or P〈0.01）.Schwann cell transplantation combined with the C5a receptor antagonist can promote the regeneration of nerve synapses in rats with spinal cord injury,and to improve their motor function,and electrophysiological function.