原位定型微囊化载体制剂在糖尿病大鼠皮肤溃疡中的作用

背景：原位定型微囊化载体制剂成分之一胰岛素可以促进溃疡愈合。目的：观察原位定型微囊化载体制剂在糖尿病大鼠皮肤溃疡中的疗效。方法：腹腔内注射链脲佐菌素建立糖尿病大鼠模型,应用外科方法建立全层皮肤缺损模型。根据皮肤溃疡处干预方式将实验动物分为4组。①空白对照组用生理盐水处理创面。②一般制剂组应用甲硝唑＋山莨菪硷1＋普通短效胰岛素处理创面。③单纯微囊组创面外敷不含有效药物成分的微囊化载体膜。④微囊化有效制剂组溃疡处外涂微囊化载体制剂膜,内含药物成分与一般制剂组相同。定时测量溃疡面积,记录溃疡愈合时间,取创面全层组织进行组织学观察,测定表皮生长因子受体、纤维连接蛋白阳性细胞数量。结果与结论：微囊化有效制剂组大鼠溃疡愈合时间短于其他3组（P〈0.05或P〈0.01）,微囊化有效制剂组表皮生长因子受体和纤维连接蛋白阳性细胞数目高于其他各组（P〈0.05或P〈0.01）。结果表明,原位定型微囊化载体制剂能够缩短愈合时间和促进糖尿病大鼠皮肤溃疡愈合。

Therapeutic effect of microencapsulation carrier preparation for in situ stereotype on skin ulcers of diabetic rats

BACKGROUND：Insulin as one of the components of microencapsulation carrier preparation for in situ stereotype can promote the ulcer healing.OBJECTIVE：To evaluate the therapeutic effect of microencapsulation carrier preparation for in situ stereotype on skin ulcers of diabetic rats.METHODS：Sprague-Dawley（SD） rats were used to establish diabetic rat models by intraperitoneal injection of streptozotocin（STZ） and to set up skin ulcer models by surgical methods.All rats were randomly divided into 4 groups by different interventions：microencapsulation preparation with effective component group（MPE）,microencasultion preparation without effective component group（MPNE）,general preparation with effective component group（GE）,blank control group（BC）.Ulcer area was measured at regular time intervals.In the first 14 days,full-thickness skin wounds were brought to make histological observation and determine the positive cell numbers expressing epidermal growth factor receptor（EGFR） and fibronectin（FN）.RESULTS AND CONCLUSION：The skin ulcer healing time of MPE group was the shortest compared with the other three groups（P 0.05 or P 0.01）.The number of EGFR and FN positive cells in MPE group was more than that of any other three groups（P 0.05 or P 0.01）.Microencapsulation carrier preparation for in situ stereotype can promote the healing course of skin ulcer in diabetic rats.