人骨软骨组织体外冷冻保存的效果

背景：异体骨软骨移植是治疗关节软骨缺损的有效方法,然而由于移植物体外有效保存时间短,限制了临床应用以及移植物的利用效率。目的：观察梯度降温冷冻保存同种异体关节软骨的保存效果。方法：将关节软骨块经体积分数10%二甲基亚砜预处理后,应用程序冷冻仪以-1℃/min行梯度降温至-4℃,-10℃,-20℃,-40℃各30min,最后至-80℃冷冻保存。结果与结论：保存1,3,6个月及1年后检测发现,与新鲜组相比,冷冻后软骨细胞存活率、细胞活性以及浅、中、深层的蛋白多糖水平下降（P〈0.05）,且软骨细胞存活率、细胞活性以及浅、中、深层的蛋白多糖水平随冷冻保存时间延长而降低（P〈0.05）,提示冷冻保存可有效地延长骨软骨移植物存活时间。

Cryopreservation effect of human osteochondral allografts in vitro

BACKGROUND：Cartilage allograft transplantation is one of effective methods for treatment of articular cartilage defects.However,the short preservation time of osteochondral allograft in vitro limits its clinical application and the utilization efficiency.OBJECTIVE：To study the preservation effect of articular cartilage allograft with controlled rate cryopreservation.METHODS：The osteochondral allografts were processed by 10% DMSO and then cooled with a controlled rate freezer at a speed of-1 ℃/min to-4 ℃for 30 minutes,to-10℃ for 30 minutes,to-20 ℃ for 30 minutes,and to-40℃ for 30 minutes.Finally,they were put into a-80 ℃ refrigerator for cryopreservation.RESULTS AND CONCLUSION：After cryopreservation for 1,3 and 6 months and 1 year,the survival rate and viability of chondrocytes and the proteoglycan level in the superficial,middle and deep layers of osteochondral allograft were decreased with time going（P 0.05） and as compared with fresh group（P 0.05） .These findings suggest that cryopreservation can effectively prolong the survival time of osteochondral allograft.