缺血预处理预防肝细胞分离过程中的缺血再灌注损伤

背景：缺血预处理能否减轻肝细胞分离过程中的缺血再灌注损伤及改善供体残留肝脏功能？经检索国内外罕见这方面的研究。目的：探讨缺血预处理对分离肝细胞及供鼠残肝缺血再灌注损伤的防治作用。方法：12只SD大鼠随机分为2组：单纯肝部分切除组和缺血预处理组,各6只。采用改良四步胶原酶灌注法分离上述切除肝脏肝细胞,同时收集术前和术后1d大鼠的血清。结果与结论：缺血预处理组切除肝脏分离肝细胞成活率、增殖活性及白蛋白合成、超氧化物歧化酶水平显著高于单纯肝部分切除组（P〈0.05）,而乳酸脱氢酶、谷丙转氨酶、丙二醛水平显著减少（P〈0.05）;与单纯肝部分切除组比较,缺血预处理组大鼠血清白蛋白、乳酸脱氢酶、谷丙转氨酶、超氧化物歧化酶、丙二醛水平差异无显著性意义（P均〉0.05）。结果表明缺血预处理能减轻肝细胞分离过程中的缺血再灌注损伤,其机制可能与其自身缺血性预适应、抗氧化、清除氧自由基的能力相关,但对供鼠肝部分切除后残肝功能的影响不明显。

Ischemic preconditioning prevents ischemia/reperfusion injury during isolation of hepatocytes

BACKGROUND： There have been few studies regarding whether ischemic preconditioning （IPC） can alleviate ischemia/reperfusion injury and improve the function of residual liver during the isolation of hepatocytes. OBJECTIVE： To investigate the preventive effects of ischemia preconditioning on hepatocyte isolation and ischemia/reperfusion injury of residual liver. METHODS： Twelve Sprague-Dawley rats were randomly and equally divided into partial hepatectomy group and IPC group. The obtained tissues were isolated by a modified four-step collagenase perfusion technique. At the same time, rat serum was collected before and 1 day after surgery. RESULTS AND CONCLUDION： Hepatocyte viability, hepatocyte proliferation, albumin （ALB） synthesis and superoxide dismutase （SOD） levels were significantly greater, while lactate dehydrogenase （LDH）, glutamate-pyruvate transaminase （GPT） and malondialdehyde （MDA） levels were significantly decreased, in the IPC group than in the partial hepatectomy group （P 0.05）. There were no significant differences in ALB, LDH, GPT, SOD and MDA levels between partial hepatectomy and IPC groups （P 0.05）. These results showed that IPC can reduce ischemia/reperfusion injury during hepatocyte isolation. The mechanism may be related to self-induced IPC and the ability of anti-oxidant and anti-oxygen free radicals. But IPC does not produce obvious effects on the function of residual liver of donor rats.