自制小牛脱蛋白松质骨与兔皮质骨来源成骨细胞的生物相容性

背景：异种脱蛋白骨与宿主骨骼具有相同的结构,抗原性较低,不会或很少引起宿主免疫反应。目的：观察自制小牛脱蛋白松质骨与兔皮质骨来源成骨细胞的生物相容性。方法：将自制小牛脱蛋白松质骨与新西兰大白兔成骨细胞复合培养,设单纯成骨细胞对照组,观察细胞生长、支架材料降解及细胞与支架材料附着情况。结果与结论：小牛脱蛋白松质骨为多孔网状,孔隙互相通连,细胞在其上附着、生长、增殖。复合培养第3天,有较多的细胞黏附并铺展在支架上;第6天,细胞在支架上完全伸展,呈长梭形;第10天细胞分泌较多基质,重叠生长并连接成网状。实验组复合培养9,12d时成骨细胞的增殖及分泌情况明显优于对照组（P〈0.05）,碱性磷酸酶活性与骨钙素表达高于对照组（P〈0.05）。表明兔皮质骨成骨细胞增殖和成骨能力强,自制的小牛脱蛋白松质骨具有骨诱导性,二者复合具有良好的生物相容性。

Biocompatibility of self-made calf deproteinized cancellous bone with rabbit cortical bone-derived osteoblasts

BACKGROUND：Heterogeneous deproteinized bone has the same structure with the host bone,and the antigen is low causing little or no host immune response.OBJECTIVE：To observe the biocompatibility of self-made calf eproteinized cancellous bone with rabbit cortical bone-derived osteoblasts.METHODS：Cells were isolated and cultured with enzyme digestion methods in vitro from the ulnar cortical bone of 3-month-old New Zealand white rabbits.Cells of the 3rd generation were attained for the following experiments.Rabbit cortical bone-derived osteoblasts were co-cultured with self-made calf eproteinized cancellous bone in vitro.Pure osteoblasts were used as controls.The cell growth,scaffold degradation and adherence condition were detected.RESULTS AND CONCLUSION：The calf eproteinized cancellous bone was porous,and its pores were connected between each other.And with the culture time extended,cells grew and proliferated rapidly.On the 3rd day of co-culture,there were some cells adhered and spread on the scaffold;on the 6th day,cells were fully extended,showing long spindle;on the 10th day,cells secreted lots of extracellular matrix,presented with overlapping growth and were connected into the network.On the 9th and 12th day,the cell proliferation and secretion in the coculture group were superior to the control group（P 0.05）;Alkaline phosphatase activity and osteocalcin level were also higher in the coculture group than the control group（P 0.05）.The self-made calf deproteinized cancellous bone had good biological compatibility with rabbit cortical bone-derived osteoblasts.