丝素蛋白/壳聚糖三维支架材料的制备方法

背景：很多研究表明丝素蛋白、壳聚糖为天然高分子材料,无毒无味,有良好的生物特性和理化性质。目的：探讨符合软骨组织工程支架材料要求的丝素蛋白/壳聚糖三维支架材料制备方法。方法：将丝素蛋白与壳聚糖按质量比分别为3∶1,1∶1,1∶3,0∶1的比例混合制备丝素蛋白-壳聚糖复合材料,通过孔径大小、孔隙率、吸水膨胀率及热水溶失率的测定,寻找丝素蛋白/壳聚糖最佳混合比例。结果与结论：丝素蛋白/壳聚糖按质量1∶1的比例混合更符合要求：孔径90～280μm,平均孔径为151.72μm;孔隙率为（92.72±4.78）%;吸水膨胀率为（141.10±6.87）%;热水溶失率交联后较交联前降低,交联前后比较差异有显著性意义（P〈0.05）。说明丝素蛋白/壳聚糖按1∶1复合支架材料符合软骨组织工程支架材料理化性质的要求,该材料有望作为软骨组织工程研究较理想的支架材料。

Preparation of three-dimensional silk fibroin/chitosan scaffolds

BACKGROUND： Many studies have shown that silk fibroin （SF） and chitosan （CS） as natural polymer materials are non-toxic and tasteless, and they have good physical and chemical properties and biological characteristics. OBJECTIVE： To discuss the preparation methods of three-dimensional SF/CS scaffolds which meet the requirements of cartilage tissue engineering scaffolds. METHODS： SF and CS were mixed at different ratios such as 3 to 1, 1 to 1, 1 to 3 and 0 to 3, then to make composite materials. In order to find out the best mixing ratio of SF and CS, the following indexes including pore size, porosity, the water absorption and loss rate in hot water were determined. RESULTS AND CONCLUSION： It was more suitable to meet the requirements when the mixed ratio of SF to CS was 1 to 1. The pore size was 90-280 μm and the average pore size was 151.72 μm. The porosity was （92.72±4.78）%. The water absorption was （92.72±4.78）%. The loss rate in hot water after crosslinking was decreased compared before crosslinking, and there was a significant difference （P 0.05）. The mixed ratio of SF to CS is at 1 to 1 meeting the physicochemical requirements of cartilage tissue engineering scaffolds. The SF/CS composite material is expected to be an ideal scaffold material for cartilage tissue engineering.