脂肪与骨髓来源间充质干细胞生物学特性的比较

背景：近几年来脂肪来源的间充质干细胞因其取材容易也被广泛研究。目的：比较脂肪来源和骨髓来源间充质干细胞的生物学特性。方法：分离及体外培养人骨髓源间充质干细胞和脂肪源间充质干细胞,比较它们的表型、细胞倍增时间及分泌因子水平等。结果与结论：脂肪来源和骨髓来源的间充质干细胞在细胞表型上类似,只有CD106的表达有差异。脂肪来源间充质干细胞增殖速率比骨髓来源的间充质干细胞快。在相同体积的脂肪组织中能够得到的干细胞前体细胞的数量是骨髓的10倍以上。提示脂肪来源和骨髓来源的间充质干细胞具有相同功能,但脂肪组织是一个更有应用前景的干细胞来源。

Comparison of the biological characteristics of bone marrow and adipose tissue derived mesenchymal stem cells

BACKGROUND： Recently, adipose tissue derived mesenchymal stem cells due to easy to be harvested have been widely studied. OBJECTIVE： To compare the biological characteristics of adipose tissue derived mesenchymal stem cells and bone marrow mesenchymal stem cells. METHODS： Adipose tissue derived mesenchymal stem cells and bone marrow mesenchymal stem cells were isolated and cultured in vitro to compare their phenotype, cell doubling time and the level of secreted factors. RESULTS AND CONCLUSION： Adipose tissue derived mesenchymal stem cells have similar phenotype and differentiation ability as bone marrow mesenchymal stem cells except CD106 expression. The frequency of mesenchymal stem cells in bone marrow and adipose tissue was also calculated and found that mesenchymal stem cells frequency in adipose tissue was 10 times higher than bone marrow. This result can be an instruction for mesenchymal stem cells clinical use.