高原低氧大鼠肺组织超微结构及其低氧诱导因子1α的表达（英文）

背景：低氧诱导因子1α可介导哺乳动物细胞适应低氧环境。目的：观察高原低氧对大鼠肺组织超微结构的影响及其低氧诱导因子1α表达变化。方法：将SD大鼠分别为进行高原低氧干预1,2,3和30d,并设置对照组。4个高原低氧组由海拔5m的西安地区途中耗时1d带到海拔2700m的青海格尔木地区、途中耗时2d带到海拔5000m的唐古拉地区,途中耗时3,30d分别带到海拔4500m的西藏那曲地区。结果与结论：光镜及电镜观察显示,急性高原低氧2d组肺组织出现明显的高原肺水肿,急性高原低氧30d组低氧诱导因子1αmRNA的表达明显增高（P〈0.01）,高原肺水肿现象则明显减轻。结果证实,低氧习服后肺组织低氧诱导因子1αmRNA表达的提高有利于减轻高原肺水肿。

Lung tissue ultrastructure and hypoxia-inducible factor 1 alpha expression in the rats exposed to high altitude hypoxia

BACKGROUND：Hypoxia-inducible factor 1α may mediate mammalian cells to adapt hypoxia environment. OBJECTIVE：To observe high altitude hypoxia on the ultrastructure of the lung tissue of hypoxia-inducible factor 1α expression. METHODS：Sprague-Dawley rats underwent high altitude hypoxia intervention for 1, 2, 3 and 30 days. Control group was set. Four high altitude hypoxia groups consisted of Golmud, Qinghai at the altitude of 2 700 m from 5-m above sea level of Xi＇an consuming 1 day, Tanggula region at 5 000 m above sea level consuming 2 days, and Tibet Nagqu at 4 500 m above sea level consuming 3 and 30 days, respectively. RESULTS AND CONCLUSION：Light and electron microscopy showed that acute high altitude hypoxia 2 day group presented apparent high altitude pulmonary edema, while the 30 day group of acute high altitude hypoxia of high altitude pulmonary edema was significantly reduced, but hypoxia-inducible factor 1α mRNA expression was significantly increased （P 0.01）. Results demonstrate that hypoxia-inducible factor 1α mRNA expression increase in lung tissues after hypoxic acclimatization can help ameliorate high altitude pulmonary edema.