组织工程肺种子细胞及其细胞外基质的研究与进展

背景：至今为止,构建组织工程肺并运用于临床尚并未取得较大进展,原因在于种子细胞来源及鉴定,移植时限选择,支撑架安全性,细胞分化程序的标准化,细胞外基质分泌的控制等多个方面都存在难点。目的：总结组织工程肺的种子细胞来源及鉴定、细胞外基质替代物的研究与进展。方法：检索中国期刊全文数据库（CNKI：1989/2011）和PubMed（1989/2011）数据库,检索词分别为＂组织工程肺,细胞源,支架,细胞外基质,体内植入＂和＂Tissue-engineered Lung,Cell Source,Support Scaffolding,lung matrices,Vivo Implantation＂,语言分别设定为中文和英文。阅读文题和摘要进行筛选,选择具有原创性,论点论据可靠且分析全面、密切相关的文章,排除重复性研究以及质量较差文章。共检索到72篇文章,按纳入及排除标准筛选后,共纳入36篇文章。结果与结论：利用合适的细胞源与支撑架进行组织工程肺研究可培育出功能健全的可用来替换的肺组织,但目前组织工程肺的研究尚无重大进展。最近研究成功运用于临床的脱细胞气管为组织工程肺治疗肺部疾病带来新的希望。

Tissue-engineered lung seed cells and extracellular matrix

BACKGROUND：There is slow progress in the reconstruction and clinical application of tissue-engineered lung because of the difficulty in the identification and utilization of cell sources,development of standardized procedures for cell differentiation,control of extracellular matrix production to meet the needs of the lung and so on.OBJECTIVE：To review the identification and utilization of cell sources and the replacement of extracellular matrix in the tissue-engineered lung research.METHODS：A computer-based online search of China Journal Full-text Database and PubMed was performed for articles and reviews in Chinese and English from 1989 to 2011.The key words were ＂tissue-engineered lung,cell source,support scaffolding,lung matrices,vivo implantation＂.Screened following reading titles and abstracts,relevant literatures were included.Repetitive articles with poor quality were excluded.A total of 72 articles were retrieved.Finally,36 literatures were included.RESULTS AND CONCLUTION：New functional replacement lung tissue can be generated through tissue engineering in the use of cell sources and related support scaffolding under a certain condition,but it is still at the basic research stage.With the development of the techniques in the tissue-engineered lung,there will be more and more new discovery.The recent clinical use and success of decellularized trachea suggests that tissue-engineered lung may be the best choice for lung diseases in the future.