体外培养人骨髓间充质干细胞向表皮细胞分化及表皮细胞角蛋白的表达

背景：动物实验已经证实骨髓间充质干细胞体外诱导可分化为表皮细胞。目的：观察体外培养条件下人骨髓间充质干细胞向表皮细胞的分化及表皮细胞角蛋白表达。方法：采用Ficoll-Paque密度梯度离心法提取人胚胎骨髓间充质干细胞,以免疫细胞化学及流式细胞仪测定细胞表面CD33、CD34标记物进行鉴定。取第3代骨髓间充质干细胞以30%条件培养基诱导其向表皮细胞分化,免疫细胞化学染色观察诱导后细胞形态与细胞角蛋白水平变化。结果与结论：采用密度梯度离心法从人胚胎骨髓中分离培养得到细胞成分均一的骨髓间充质干细胞。骨髓间充质干细胞经体外诱导后,出现细胞角蛋白19表达阳性细胞,说明骨髓间充质干细胞在体外诱导后可能发生向表皮细胞分化。

Mesenchymal stem cells can differentiate to epidermal cells and express keratins in vitro

BACKGROUND：Animal experiments have proved that bone marrow mesenchymal stem cells（MSCs） can mostly differentiate into epidermal cells.OBJECTIVE：To investgate the feasibility of inducing MSCs into epidermal cells and the expression of keratins.METHODS：MSCs were extracted by Ficoll-Paque density gradient centrifugation.The expressions of CD34 and CD44 in MSCs cultured for three passages were deteced by immunocytochemical staining and flow cytometric examinations.The third passage MSCs were culured in 30% conditioned medium and induced to differentiate into epidermal cells.The changes,of cytokeratin 19 were detect by immunocytochemical staining and the morphological features were observed after being induced.RESULTS AND CONCLUSION：The MSCs extracted by Ficoll-Paque density gradient centrifugation were pure.After being induced with conditioned medium,cytokeratin 19 in MSCs were observed,indicating that MSCs may be induced into epidermal cells.