静磁场作用下金属离子对成骨细胞的毒性

背景：体内体外实验研究证明,静磁场具有促进成骨细胞分化及骨形成的作用。目的：观察静磁场作用下金属离子对成骨细胞毒性的影响。方法：将CoCl2粉末与CrCl3粉末溶于无菌注射用水配制成CoCl2溶液和CrCl3溶液,将金属离子Co2＋、Cr3＋分别与小鼠颅骨成骨细胞（MC3T3-E1）在不同的磁场强度（1,10,100mT）下共培养。实验分4组：设Co2＋＋Cr3＋组（对照组）和Co2＋＋Cr3＋分别＋1,10,100mT组。结果与结论：恒磁场作用下的成骨细胞呈现更加成熟的形态学特征,Co2＋、Cr3＋金属离子对成骨细胞MC3T3的毒性明显降低;同时G2M（分裂期）分布比例明显增加,细胞停滞在G0G1（休眠期）的比率明显减少;与对照组相比,不同场强磁场加载后,成骨细胞碱性磷酸酶活性明显增强（P〈0.05）。提示一定强度的静磁场可拮抗Co2＋、Cr3＋金属离子对成骨细胞的毒性作用。

Static magnetic field reduces the toxic effect of metal ions on osteoblasts

BACKGROUND：Static magnetic field can promote the proliferation,differentiation and functional activity of osteoblasts.OBJECTIVE：To investigate the toxic effect of metal ions Co2＋,Cr3＋ on osteoblasts under the static magnetic field.METHODS：CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water.Mouse calvarial osteoblasts（MC3T3-E1） were cultured with Co2＋ and Cr3＋ ions in different magnetic field of 1,10,100 mT.Cells only cultured with Co2＋ and Cr3＋ ions were taken as control group.RESULTS AND CONCLUSION：Under the static magnetic field,osteoblasts showed more mature morphological features;the toxic effect of metal ions Co2＋ and Cr3＋ on osteoblasts reduced significantly;G2M（dividing phase） distribution ratio increased and G0G1（dormancy stage） distribution ratio decreased;the A value tested by alkaline phosphatase activity in the three experiment groups up-regulated obviously.The static magnetic field in certain intensity could antagonize the toxic effect of metal ions Co2＋ and Cr3＋ on osteoblasts.