深低温冷冻骨的形态学及免疫组织化学检测

背景：深低温冷冻技术能够降低同种异体骨的免疫原性,对修复骨缺损具有重要意义。目的：观察深低温冷冻对骨材料中骨形态发生蛋白2表达的影响。方法：18只新西兰兔随机分为3组,深低温冷冻3个月组,深低温冷冻6个月组,新鲜骨对照组,取右侧胫骨中上1/3,分别于-80℃保存保存3个月,6个月及不保存,进行苏木精-伊红染色和骨形态发生蛋白2免疫组织化学染色。结果与结论：深低温冷冻后骨材料形态学,与新鲜骨差异不大;深低温冷冻3个月及6个月骨组织中骨形态发生蛋白2均有表达,但较新鲜骨降低。

Morphological and immunohistochemical detection of deep-frozen bone

BACKGROUND： Bone morphogenetic protein 2 （BMP-2） in fracture healing and repair process plays an important role in deep-freezing technology. OBJECTIVE：To investigate the effects of deep-freezing technology on BMP-2 expression in the bone materials. METHODS：18 rabbits were randomly and evenly divided into three groups：deep-frozen 3 months, deep frozen 6 months, fresh bone. The middle upper 1/3 right tibia from each group was taken and preserved at -80 ℃ for 3 or 6 months or was not preserved. Then all bone samples were performed hematoxylin-eosin staining and BMP-2 immunohistochemical staining. RESULTS AND CONCLUSION：There was no significant difference in morphology between deep frozen bone and fresh bone. BMP-2 was expressed in bone deep-frozen for 3 and 6 months, but the expression was lower than that in the fresh bone.