不同浓度富硒温泉水对增生性瘢痕成纤维细胞增殖的影响

背景：临床研究证实,富硒温泉水对增生性瘢痕有明显的抑制作用。目的：观察富硒温泉水对人增生性瘢痕成纤维细胞增殖的影响。方法：组织块法体外培养人增生性瘢痕成纤维细胞,取第3~5代细胞,用含10%,20%,30%富硒温泉水及自来水和蒸馏水的培养基培养48h,测细胞增殖情况。结果与结论：富硒温泉水培养的人增生性瘢痕成纤维细胞数量减少,突起不同程度的变短或者缺失,细胞质及胞核浓缩。不同浓度的富硒温泉水、自来水、蒸馏水均对成纤维细胞的增殖有抑制作用,以富硒温泉水培养基的抑制作用最明显（P〈0.05）,且随浓度的升高,抑制率增大。说明富硒温泉水可以剂量依赖性地抑制人增生性瘢痕成纤维细胞的增殖。

Effects of different doses of selenium-rich water on the proliferation of hypertrophic scar fibroblasts

BACKGROUND：Clinical studies have shown that selenium-rich water can obviously inhibit hypertrophic scar. OBJECTIVE：To investigate the effects of selenium-rich water on the proliferation of human hypertrophic scar fibroblasts （HSFBs）. METHODS：Human HSFBs were cultured by the tissue explants method. Passages 3-5 human HSFBs were cultured with 10%, 20% and 30% selenium-rich water, running water and distilled water for 48 hours. Cell proliferation was measured by MTT method. RESULTS AND CONCLUSION：Human HSFBs cultured with selenium-rich water were reduced in number, cell processes were shortened to different extents or lacked, and cytoplasm and nucleus were reduced. Different doses of selenium-rich water, running water, and distilled water exhibited inhibitory effects on the proliferation of human HSBFs, in particular selenium-rich water and in a dose-dependent manner（P 0.05）.Results showed that selenium-rich water inhibits the proliferation of human HSFBs in a dose-dependent manner.