| 鼠细胞角蛋白EndoB基因组DNA克隆No4的亚克隆及核苷酸序列分析 |
| |
| 引用本文： | 张凤蕴.鼠细胞角蛋白EndoB基因组DNA克隆No4的亚克隆及核苷酸序列分析[J].哈尔滨医科大学学报,1989,23(6):388-391. |
| |
| 作者姓名： | 张凤蕴 |
| |
| 作者单位： | 哈尔滨医科大学免疫学教研室 |
| |
| 摘 要： | 本文报道了从鼠细胞角蛋白基因组DNA文库中,经核酸分子杂交筛选的与鼠细胞角蛋白Endo B同源的阳性克隆No.4的亚克隆及核苷酸序列分析,又经微机辅助分析,证明No.4的461个核苷酸序列中,与Endo B cDNA同源性达93%,是与Endo B cDNA相关的假基因。
|
| 关 键 词： | 细胞角蛋白 EndoB 核苷酸序列 |
Subcloning and Sequencing of Genomic DNA Clone No.4 of Mouse Cytokeratin Endo B |
| |
| Zhang Fengyun.Subcloning and Sequencing of Genomic DNA Clone No.4 of Mouse Cytokeratin Endo B[J].Journal of Harbin Medical University,1989,23(6):388-391. |
| |
| Authors: | Zhang Fengyun |
| |
| Institution: | Department of Immunology |
| |
| Abstract: | Four clones No.23, No. 4, No. 10, No.14 were screened from genomic DNA library encoding cytokeratin by hybridization. The DNA digested fragments with different orientation of clone No. 4 were subcloned into M_(13) phage vector, then sequenced by the dideoxynucleotide sequencing method. The nucleotide sequnce was analyzed and compared with Endo B structure DNA (clone No.23) and cDNA sequences using microcomputer. There aren't introns in No. 4. The overall homology is 93% between clone No. 4 and cDNA of Endo B for 461_(bp). However, one deletion in the 461_(bp) resulted in a serious frame shift, so No. 4 is a processed pseudogene corresponding to Endo B cDNA. |
| |
| Keywords: | Cytokeratin Endo B Mucleotide sequence Pseudogene |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
