microRNA143?microRNA145对胆管癌细胞株QBC939生长周期及凋亡的影响

目的:探讨microRNA143、microRNA145与肝外胆管癌的关系以及microRNA143、microRNA145对胆管癌细胞株QBC939细胞周期及凋亡的影响。方法:提取11对肝外胆管癌组织标本和正常胆管组织标本的RNA,实时荧光定量PCR(realtimePCR)检测microRNA143、microRNA145的表达;将microRNA143、microRNA145质粒转染人胆管癌细胞株QBC939,分别以流式细胞仪和噻唑蓝(MTT)的方法检测转染前后细胞周期、凋亡及生长情况。结果:相对正常胆管组织,胆管癌组织microR-NA143、microRNA145表达率分别为0.048、0.035(P<0.05)。较对照质粒组,microRNA143组、microRNA145组G1期细胞百分比明显增多[(66.08%±1.93%、65.57%±1.85%)vs(42.87%±1.37%)]、S期细胞百分比明显减少[(19.16%±1.15%、21.40%±1.65%)vs(38.76%±1.83%)]、细胞凋亡率增加[(18.49%±2.26%、16.89%±2.47%)vs(4.68%±1.65%...

Effects of microRNA143 and microRNA145 transfection on cell cycle and apoptosis of cholangiocarcinoma cell line QBC939

Objective:To explore the effects of microRNA143,microRNA145 transfection on biological behaviors of cholangiocarcinoma cell line QBC939,and the relationship between microRNA143,microRNA145 and extrahepatic cholangiocarcinoma.Methods:RNA of 11 pairs of specimens of extrahepatic cholangiocarcinoma and normal bile duct was extracted,and then the expression of microRNA143 and microRNA145 was detected by real-time PCR.Expression plasmid of microRNA143(or microRNA145) was transiently transfected into human cholan...