中医药对各型慢性阻塞性肺疾病大鼠核因子κB和γ-谷氨酰半胱氨酸合酶表达的影响

目的 通过观察慢性阻塞性肺疾病（COPD）大鼠核因子κB和γ-谷氨酰丰胱氨酸合酶（γ-GCS）水平的变化，探讨中医药治疗COPD的机理。方法 采用改良烟熏加气管滴加脂多糖方法复制COPD模型，以免疫组化法检测核因子κB和γ-GCS水平：二次造模后，各治疗组分别灌服相应证型的中药合剂，连续14天。寒痰蕴肺治疗组灌服小青龙汤煎剂，痰热阻肺治疗组大鼠灌服麻杏石甘汤煎剂，肺气虚治疗组灌服王屏风散煎剂，脾气虚治疗组灌服六君子汤煎剂，肾气虚治疗组灌服人参蛤蚧散煎剂，模型组大鼠每天给与2mL.生理盐水灌胃。空白对照组不做任何干预。结果与空白对照组相比，各模型组γ-GCS、NF-κB在支气管和肺泡上皮等都为高表达；经过中药治疗后，阳性表达率有明显降低，其差异均有显著性（P〈0．05），结论 通过辨证论治各中药方剂能纠正COPD大鼠的氧化／抗氧化失衡，减少炎性反应，从而有效治疗COPD。

Effects of TCM treatment according to syndrome differentiation on expressions of nuclear factor-kappaB and gamma-glutamylcysteine synthetase in rats with chronic obstructive pulmonary disease of various syndrome types]

OBJECTIVE: To explore the mechanism of traditional Chinese medicine (TCM) treatment according to syndrome differentiation in treating chronic obstructive pulmonary disease (COPD) by observing the changes of nuclear factor-kappaB (NF-kappaB) and gamma-glutamylcysteine synthetase (gamma-GCS) expression levels in rats. METHODS: COPD model was established by modified method of combining fumigation and lipopolysaccharide (LPS) intra-tracheal dripping. Model rats were treated respectively for succesive 14 days according to their syndrome, that is, Xiaoqinglong Decoction to the rats of cold-phlegm accumulation in Fei, Maxing Shigan Decoction to those of heat-phlegm accumulation in Fei, Yupingfeng Decoction to those of Fei-qi deficiency, Liujunzi Decoction to those of Pi-qi deficiency, Renshen Gejie Decoction to those of Shen qi-deficiency. Besides, model rats in the model control group received 2mL normal saline daily, and no intervention was applied in the normal control group. The expression of gamma-GCS and NF-kappaB was detected by immunochemistry before and after treatment. RESULTS: Compared with that in the normal rats, the expressions of gamma-GCS and NF-kappaB in bronchial and alveolar epithelium of COPD rats before treatment were significantly higher, but the positive expression rates were lowered after treatment significantly (P<0.05). CONCLUSION: TCM treatment according to syndrome differentiation could rectify imbalance of oxidation/anti-oxidation and alleviate inflammatory reaction in COPD rats, thus to treat COPD effectively.