自噬在骨肉瘤化疗中作用的体内研究

目的:探讨自噬的调节对骨肉瘤化疗的影响。方法:建立裸鼠骨肉瘤动物模型,自噬抑制剂3甲基腺嘌呤(3MA)和顺铂(DDP)干预肿瘤的生长,观察不同药物对骨肉瘤的生长情况的影响,检测肿瘤大小变化,HE染色观察病理表现。结果:对照组、DDP组、3MA+DDP组3组之间用药前后体积的比较差异具有统计学意义。DDP确实能较有效的抑制肿瘤的生长,加入3MA干预后能增强其敏感性,肿瘤的生长得到了进一步的抑制。HE染色见3MA+DDP组移植瘤组织中明显的坏死区。结论:3MA联合DDP化疗能提高对骨肉瘤细胞的杀伤率。

The Role of Autophagy in Chemotherapy for Osteosarcoma in vivo

Objective：To investigate the effects of regulation of autophagy on the chemotherapeutic sensitivity in osteosarcoma in vivo.Methods：The animal models of osteosarcoma in nude mice were established.3MA and cisplatin（DDP） were injected into the animals.The growth of osteosarcoma was observed by its size.The pathologic manifestations were detected by HE staining.Results：There were significant differences among the control group,DDP group and 3MA＋DDP group.DDP could effectively inhibit tumor growth.And 3MA could enhance the sensitivity of DDP.HE staining showed that there was a clear area of necrosis in the transplanted tumor tissue in 3MA＋DDP group.Conclusion：The combination of 3MA and DDP can increase cells death than exposure to DDP alone in osteosarcoma.