玉红膏促进软组织损伤修复过程中相关因子表达的实验研究

目的:观察中药玉红膏对家兔的软组织损伤模型中创面肉芽组织成纤维细胞生长因子(b-FGF)及转化生长因子(TGF-β1)表达的影响,从分子水平探讨中药外用制剂促进软组织修复的机制。方法:将30只家兔采用经典的全层皮肤切割伤造模,并随机分为3组。治疗组外敷玉红膏并包扎,清洁换药组常规清洁换药,空白组仅换敷料。收集不同时期的肉芽组织,高倍镜下进行病理切片观察,用SABC法检测创面中b-FGF及TGF-β1蛋白表达,在400倍光镜下观察,BI-2000图像分析系统分析,结果以平均灰度值表示。结果:在同一取材的条件下,3组b-FGF蛋白表达在创伤后6d存在差异(P0.05)。TGF-β1蛋白表达在创伤后10,14d存在差异(P0.05)。同时,肉芽组织的生长状况在同一时期取材时间的条件下存在组间不同,在不同取材时间的条件下存在组内差异。结论:本实验证明中药玉红膏能够通过刺激创面内细胞产生内源性生长因子,进而促进创面的修复。

Effects of Yuhong Ointment on Promoting the Expression of b-FGF and TGF-β1 in the Repair of Soft Tissue Injuries

Objective：To study the effects of Yuhong Ointment on the expression of basic fibroblast growth factor （b-FGF） and transforming growth factor-β1 （TGF-β1） in granulation tissues of rabbit models with soft tissue injuries, so as to explore mechanism of external Chinese drugs for repairing of soft tissues in molecular levels. Methods：The rabbit models were established by classical method of full thickness skin wounds, and then divided into 3 groups randomly. The rabbits in the experimental group were treated with spread of Yuhong Ointment at the wound, the rabbits in clean group were treated with routine dressing changes and disinfection, and the rabbits in the control group were treated with dressing changes only. The granulation tissues in different stages were collected and observed with high power microscope. The expression of TGF-β1 and b-FGF in wound tissues were detected using StreptA ridin Biotin Complex （SABC） method. Results：The results showed that the expression of b-FGF was significantly different among 3 groups at the 6th day after trauma （P0.05）. TGF-β1 expression was also significantly different among 3 groups at the 10th and 14th days after trauma （P0.05）. At the same time, the growth states of granulation tissues were different among 3 groups at the same trauma stage, and within the same group among different trauma stages. Conclusion：This experimental study shows that Yuhong Ointment can effectively promote the repair of soft tissues after trauma by stimulating production of endogenous growth factor from cells in wound.