影响内皮细胞凋亡的因素及其机制探讨

目的：观察亚砷酸钠（Ars)，内毒素（LPS）、肿瘤坏死因子（TNF）、烧伤血清、创伤血清对内皮细胞凋亡的影响及其机制。方法：体外培养内皮细胞ECV－304，与上述物质孵育24小时后检测凋亡水平及ICAM－1.bcl-2,p53表达的变化，结果：Ars可使内皮细胞发生剂量依赖的凋亡增加（与对照相比，P＜O．01），但是LPS、TNF烧伤血清和创伤血清对内皮细胞的作用有限，细胞凋亡率在15％以下。除10％烧伤血清外，所有处理因素都内皮细胞ICAM－1表达降低。体外诱导内皮细胞凋亡与bcl-2表达降低、p53表达增多有关，结论：Ars,LPS,TNF烧伤血清和创伤血清通过下调bcl-2表达，上调p53表达来影响内皮细胞凋亡，并且抑制内皮细胞功能。

Substances That Induced Endothelial Cell Apoptosis and the Mechanisms Involved

Objective: To investigate the endothelial cell apoptosis inducing effect of substances, including sodium arsenite (Ars), lipopolysaceharide (LPS), tumor necrosis factor (TNF), burned serum and traumatic serum and the mechanisms involved. Methods: By comparing the changes of apoptosis rates and the expressions of ICAM -1, bc1 - 2, p53 after endothelial cells ECV - 304 were incubated with these apoptosis inducing substances. Results: Ars increased the apoptosis rate of endothelial cells and this effect was dose-dependent (compared with control, P<0. 01. LPS, TNF, burned serum and traumatic serum had only limited effect on endothelial cell apoptosis, the apoptosis rate was below 15% Except for 10% burned serum, all these substances decreased the expression of endothelial cell ICAM - 1, in vital en- dothelial cell apoptosis inducing effect were correlated to the decrease in the expression of bcl - 2 and the increase in the expression of p53 and ICAM - 1. Conclusion: Ars, LPS, TNF, burned serum and traumatic serum influenced endothelial cell apoptosis through down - regulation of bcl - 2 and up - regulation of p53 and inhibited their functions.