督脉电针对早期受损伤的脊髓神经营养素-3表达的影响

目的 观察督脉电针对早期受损伤的脊髓神经营养素-3(NT-3)表达的影响及其细胞定位.方法 成年雌性大鼠分为损伤组和电针加损伤组.全横断损伤两组大鼠的脊髓1d后,开始对电针+损伤组大鼠进行督脉电针,损伤组大鼠不做督脉电针.电针加损伤组在电针后1、3和7d时间点取出脊髓损伤区组织,损伤组也在相应时间点取出损伤区组织,用ELISA方法检测损伤区组织NT-3水平.再取电针后7d大鼠脊髓损伤区及其邻近组织切片做NT-3的免疫荧光组织化学双标染色.结果 电针加损伤组和损伤组的脊髓损伤区组织NT-3表达在时间上基本一致;在前3 d,NT-3水平是下降的,在后3 d,NT-3水平是增高的.但是,与损伤组相比,电针加损伤组的NT-3水平是明显增高(P＜0.05).免疫荧光双标染色结果显示,神经元、星形胶质细胞、少突胶质细胞和巨噬细胞都有NT-3的表达.结论 督脉电针可以促进受损伤早期的脊髓组织细胞合成和分泌内源性NT-3,这可能是督脉电针促进急性脊髓损伤修复的适宜微环境因素之一.

Effects of Governor Vessel electro-acupuncture on expression of neurotrophin-3 in rat spinal cord injured early

Objective To observe the effects of Governor Vessel(GV) electro-acupuncture on the expression of neurotrophin-3(NT-3) in rat spinal cord injured early and the cellular localization of NT-3.Methods Adult female SD rats were divided into the injury group and the electro-acupuncture+injury group.One day after complentely transected spinal cord of rats of two groups,GV electroacupuncture was carried out for rats of the electro-acupuncture+injury group,but no for rats of the injury group.The tissues of the injury area of spinal cord in the electro-acupuncture + injury group were collected at 1 d,3 d and 7 d after electro-acupuncture.The tissues of the injury area of spinal cord in the injury group were collected at same time points.NT-3 level of the tissues of both groups was detected by ELISA method.In addition,double-label immunofluorescence was done for the tissue sections of the injury area of spinal cord and nearby the injury area at 7 d after electro-acupuncture.Results NT-3 expression in the tissue of the injury area of spinal cord was similar on time points in the electro-acupuncture + injury and injury groups.NT-3 level was decreased from the establishment of spinal cord transection model to 3 d after surgery and was increased at 7 d after surgery.However,NT-3 level in the electro-acupuncture+injury group was higher than that in the injured group(P < 0.05).The result of double-label immunofluorescence showed that neurons,astrocytes,oligodendrocytes and macrophages were NT-3 positive staining.Conclusion GV electro-acupuncture may enhance the synthesis and secretion of endogenous NT-3 for the tissue cells of spinal cord injured early,which may be one of the important factors in the appropriate microenvironment created by GV electro-acupuncture to promote the recovery of acute spinal cord injury.