丝裂原活化蛋白激酶介导人胚肺成纤维细胞增生的观察

目的探讨丝裂原活化蛋白激酶（ｍｉｔｏｇｅｎａｃｔｉｖｉｔｅｄｐｒｏｔｅｉｎｋｉｎａｓｅ，ＭＡＰＫ）在６０Ｃｏγ射线照射后人胚肺成纤维细胞（ｈｕｍａｎｅｍｂｒｙｏｌｕｎｇｆｉｂｒｏｂｌａｓｔ，ＨＥＬＦ）增生中的作用及其与血管紧张素Ⅱ（ａｎｇｉｏｔｅｎｓｉｎⅡ，ＡⅡ）的关系。方法对培养的ＨＥＬＦ进行１～５戈瑞（Ｇｙ）的６０Ｃｏγ－射线照射，用酶标方法检测细胞增生，用免疫细胞化学结合图像分析检测ＡⅡ、ＭＡＰＫ和Ⅰ型前胶原合成以及硝普钠抑制ＡⅡ的作用。结果１～５Ｇｙ照射能促进细胞增生及Ⅰ型前胶原合成，受照射细胞ＡⅡ和ＭＡＰＫ合成增加，外源性ＡⅡ能促进细胞增生而硝普钠则能抑制ＡⅡ的作用。结论６０Ｃｏγ－射线照射促进ＨＥＬＦ增生是一种链锁反应过程，ＡⅡ和ＭＡＰＫ介导了这一反应，ＭＡＰＫ在细胞增生信号传导中可能起着限制性阀门的作用。

Observation on human embryonic lung fibroblast proliferation mediated by mitogen activated protein kinase

OBJECTIVE: To study the effect of mitogen activated protein kinase (MAPK) on the proliferation of human embryonic lung fibroblast (HELF) induced by 60Co irradiation and the relationship between MAPK and angiotensin II (A II). METHODS: Cell proliferation was measured with enzyme-labeling method; angiotensin II, MAPK and type I precollagen synthesis as well as the role of sodium nitroprusside on the inhibition of A II were evaluated adopting immunohistochemical technique combined with image analysis after 1-5 Gy 60Co gamma-ray irradiation on the cultured HELF. RESULTS: Irradiation at 1-5 Gy promoted cell proliferation and type I precollagen synthesis; the syntheses of A II and MAPK were also increased in the irradiated cells. Exogenous A II enhanced cell proliferation, but sodium nitroprusside inhibited the action of A II. CONCLUSIONS: HELF proliferation induced by 60Co irradiation is a chain reactive course, in which, A II and MAPK are involved. MAPK may play a role of limiting valve in the signal transduction of cellular proliferation.