复方甘草酸苷对小鼠暴发性肝衰竭Fas/FasL介导的肝细胞凋亡的保护作用

Effect of SNMC on inhibiting hepatocyte apoptosis induced by Fas/FasL in mice with fulminant hepatic failure

AIM: To study the effect of Stronger Neo-Minophagen C (SNMC) on inhibiting hepatocyte apoptosis in mice with fulminant hepatic failure (FHF) and its mechanism. METHODS: Seventy mice were divided randomly into three groups: A (normal control group, n - 5), B (model group, n = 5) and C (SNMC-protecting group, n = 60). Mice in groups A and B were killed 6 h after treated with SNMC. Mice in group C were killed 6 h, 1, 3, 5 and 7 d after treated with SNMC respectively, five mice for each time. The hepatocyte apoptosis in situ was detected by TUNEL. The ultrastructure of hepatocytes was examined by electron microscopy. The expression of Fas, FasL and caspase-3 was detected by immunohistochemistry. RESULTS: The hepatocyte apoptosis index was 32.3% 6 h after treated with SNMC in group C, which was decreased to 5% after 7 d. The apoptosis index was significantly lower 1, 3, 5 and 7 d after treated with SNMC in group C than that in group B (P<0.01). Small amount of Fas and caspase-3 were expressed in group A, while no FasL was expressed. The cells with Fas, FasL and Caspase-3 expression were increased significantly. However, Fas, FasL and Caspase-3 expression was decreased with the increasing of time in group C, and their levels were significantly lower 1, 3, 5 and 7 d after treated with SNMC than those in group B (P<0.01). CONCLUSION: SNMC can effectively protect hepatocytes from apoptosis in mice with FHF, and the mechanism may be related to its effect on the inhibition of Fas/FasL.