川芎嗪对大鼠脑缺血再灌注Bcl-2、c-fos、Caspase-3的影响

目的:探讨川芎嗪干预脑缺血再灌注损伤后对Bd-2蛋白、C-fos蛋白、Caspase-3蛋白的表达影响及其保护大脑损伤的作用机制.方法:将SD大鼠30只,随机分为3组,即假手术组、模型组和治疗组,每组10只.建立脑缺血再灌注模型.治疗组用川芎嗪干预,采用免疫组化法测定川芎嗪干预大鼠脑缺血24 h后对Bd-2蛋白、C-fos蛋白、Caspase-3蛋白表达的变化.结果:在脑缺血再灌注损伤24 h后,治疗组脑组织Bcl-2蛋白阳性神经元数较模型组明显增多,差异有显著性意义(P＜0.01);治疗组C-fos蛋白和Caspase-3蛋白表达阳性神经元数较模型组明显减少,差异有显著性意义(P＜0.01).结论:川芎嗪能上调Bcl-2蛋白和下调C-fos蛋白和Caspase-3蛋白表达,川芎嗪可能通过抑制脑缺血再灌注损伤后细胞凋亡机制,从而对脑脑缺血再灌注损伤有保护作用.

The Effect of Tetramethylpyrazine on Bcl-2、c-fos、Caspase-3 in Rats Cerebral Ischemia Reperfusion

Objective:To explore the effect of tetramethylpyrazine on the expression of Bcl-2,c-fos and Caspase-3 proteins and protective cerebral mechanism after cerebral ischemia/reperfusion.Methods:Thirty SD rats were selected and randomized into three groups:sham group,model group and treatment group,each involved ten rats.The cerebral ischemia reperfusion models were established.The expression of Bcl-2,c-fos and Caspase-3 proteins were detected using immunohistochemical method 24 h after cerebral ischemia/reperfusion treated by tetramethylpyrazine in the treatment group.Results:The expression of Bcl-2 proteins in the treatment group were significantly higher than those in the model group(P<0.01).While the expression of c-Fos and Caspase-3 proteins in the treatment group were significantly lower than those in the model group(P<0.01).Conclusion:tetramethylpyrazine can upregulated the expression of Bcl-2 protein and downregulated the expression of c-Fos and Caspase-3 proteins.tetramethylpyrazine may protect the cerebral effect on ischemia reperfusion injury through inhibiting neuron apoptosis.