HPLC法测定他克莫司滴丸的含量 |
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引用本文: | 梁颖,王俊杰,刘涛.HPLC法测定他克莫司滴丸的含量[J].疾病监测与控制杂志,2012(9):531-532. |
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作者姓名: | 梁颖 王俊杰 刘涛 |
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作者单位: | [1]内蒙古医科大学附属人民医院,内蒙古呼和浩特010020 [2]内蒙古医科大学药学院,内蒙古呼和浩特010059 |
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摘 要: | 目的建立他克莫司滴丸的含量测定方法。方法采用高效液相色谱法,色谱柱为Hypersil ODS(4.0mm×250mm,5μm),流动相为乙腈-水(60:40),柱温为50℃,流速1.0mL.min-1,检测波长为220nm;对3批他克莫司滴丸进行含量测定。结果他克莫司浓度在100~800μg.mL-1范围内,峰面积与浓度线性关系良好,平均回收率分别为99.92%(n=9),RSD为0.69%,3批样品中他克莫司的含量分别为99.76%、99.79%、99.93%。结论本法测定他克莫司的含量,简便快捷、结果准确、重现性好、易于操作,可用于他克莫司滴丸中他克莫司含量的测定。
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关 键 词: | 他克莫司滴丸 HPLC 含量测定 |
Determination of tacrolmius in tacrolmius dropping pills by HPLC |
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Authors: | LIANG Ying WANG Jun-jie LIU Tao |
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Institution: | .(Affiliated People Hospital,Inner Mongolia Medical College,Hohhaot 010020,China) |
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Abstract: | Objective To establish HPLC method for determination of tacrolimus in tacrolimus dropping pills.Methods HPLC method was performed on Hypersil ODS(4.0mm×250mm,5μm),with acetonitrile-water(60:40) as mobile phase,the column temperature was 50℃,the flow rate was 1.0mL?min-1,and the UV detection wavelength was set at 220 nm.3 batch of samples were detected.Results Tacrolimus content has a linear range of 100~800μg?mL-1with a mean recovery of 99.92%(n=9,RSD=0.69%).Conclusion The method appeared to be simple,rapid,accurate and repeatable and easy to operate,it could be used for determination of tacrolimus in tacrolimus dropping pills. |
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Keywords: | Tacrolimus dropping pills HPLC Content Determination |
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