小鼠骨髓内皮细胞对脐血造血细胞体外扩增的作用

目的：观察骨髓内皮细胞对脐血造血细胞体外扩增的影响。方法：体外培养骨髓内皮细胞株细胞，收集无血清条件培养液（命名为ECM）。用MiniMACS磁珠法分离脐血CD34+细胞。检测CD34+细胞经ECM体外液体培养24 h后或与骨髓内皮细胞层(ECL)共培养24 h后脐血造血细胞的扩增倍数，并观察在加入bFGF的培养条件下收集的骨髓内皮细胞条件培养液（bFGF-ECM）是否能加强对脐血造血细胞的扩增作用。 结果：ECM，ECL 皆能显著扩增脐血早期或晚期造血细胞，且二者对早期造血细胞的扩增效果是类似的；bFGF-ECM对脐血造血细胞的扩增倍数明显高于用ECM扩增的倍数。结论：小鼠骨髓内皮细胞对脐血造血细胞有明显的体外扩增作用。

Effects of murine bone marrow endothelial cells on the in vitro expansion of human cord blood hematopoietic progenitor cells

Objective To observe the effects of bone marrow endothelial cells on the expansion of human umbilical cord blood (UCB) hematopoietic progenitors in vitro.Methods Bone marrow endothelial cells were cultured and the serum free conditioned media (ECM) were collected.CD34 + cells derived from human umbilical cord blood (UCB) were isolated by MiniMACS.Hematopoietic progenitor cells were assayed after culturing with ECM or co culturing with endothelial cell layer (ECL) for 24 hours.The effects of conditioned medium of endothelial cells which were cultured with bFGF (bFGF ECM) on the expansion of cord blood hematopoietic progenitors also were investigated. Results UCB hematopoietic progenitors were significantly expanded by adding ECM or co cultured with ECL,and ECM cultures and ECL cultures showed similar expansion effect on early progenitor cells.The effect of bFGF ECM on the expansion of UCB hematopoietic progenitors was significantly better than that of ECM. Conclusion Murine bone marrow endothelial cells support the quantitative expansion of UCB hematopoietic progenitor cells in vitro.