| 甲基茉莉酮酸对悬浮培养南方红豆杉细胞代谢的影响 |
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| 引用本文： | 王艳东,路明,元英进. 甲基茉莉酮酸对悬浮培养南方红豆杉细胞代谢的影响[J]. 中草药, 2003, 34(1): 27-30 |
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| 作者姓名： | 王艳东 路明 元英进 |
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| 作者单位： | 天津大学化工学院,制药工程系,天津,300072 |
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| 基金项目： | 国家自然科学基金 (2 0 1760 3 8)，杰出青年科学基金 B(2 0 0 2 860 7) |
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| 摘 要： | 目的 探讨甲基茉莉酮酸作用下悬浮培养南方红豆杉细胞的系列生理生化反应。方法 TTC92，3，5-triphenyltetrazolium chloride)和蛋白含量测定方法以及酶活分析技术。结果 甲基茉莉酮酸不仅强烈抑制细胞活力，使细胞初生代谢受到显著抑制，并且还诱导PAL（苯丙氨酸解氨酶）活性增强，在一定程度上抑制了PPO（多酚氧化酶）的活性，使胞外酚含量增加且极大峰比对照提前约3d出现。结论 甲基茉莉酮酸不仅促进细胞由初生代谢提前向次生代谢转化，并且能够增强细胞的次生代谢，有利于细胞相关次生代谢产物的合成。
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| 关 键 词： | 细胞代谢 南方红豆杉 紫杉醇 甲基茉莉酮酸 悬浮培养 |
| 文章编号： | 0253-2670(2003)01-0027-04 |
| 收稿时间： | 2002-05-27 |
| 修稿时间： | 2002-05-27 |
Cell metabolism of Taxus chinensis var. mairei in suspension cultures treated with methyl jasmonate |
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| WANG Yandong,LU Ming and YUAN Yingjin. Cell metabolism of Taxus chinensis var. mairei in suspension cultures treated with methyl jasmonate[J]. Chinese Traditional and Herbal Drugs, 2003, 34(1): 27-30 |
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| Authors: | WANG Yandong LU Ming YUAN Yingjin |
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| Affiliation: | Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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| Abstract: | Object To study the physiological changes of Taxus chinensis var. mairei (Lemee et Lévl.) Cheng et L. K. Fu in the case of methyl jasmonate (MJ). Methods TTC assay, soluble protein measurement and enzyme analysis were used. Results It was observed that MJ inhibited Taxus cell growth in the view of primary metabolism. MJ induced phenylalanine ammonia lyase(PAL) activity while it inhibited polyphenoloxidase (PPO) activity. Extracellular phenolic content after addition of MJ increased to the maximum at the three days than that of the control group. Conclusion It was demonstrated that MJ induced the transition of Taxus cell from primary metabolism to secondary metabolism. This is favorable for secondary metabolism of Taxus cells. It is important to study the physiology of Taxus cells for revealing the mechanism of MJ. |
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