| 登革病毒cDNA的生物信息学分析及其Oligo探针设计 |
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| 引用本文： | 肖维威,马文丽,马晓冬,毛向明,郑文岭.登革病毒cDNA的生物信息学分析及其Oligo探针设计[J].南方医科大学学报,2003,23(9):905-907. |
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| 作者姓名： | 肖维威 马文丽 马晓冬 毛向明 郑文岭 |
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| 作者单位： | 1. 第一军医大学分子生物学研究所,广东,广州,510515
2. 广州军区广州总医院分子肿瘤学研究所,广东,广州,510010 |
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| 基金项目： | 国家自然科学基金(39880032)，广州市重点科技攻关项目(99-Z-022-01)~~ |
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| 摘 要： | 目的设计登革病毒诊断芯片的Oligo探针。方法利用BLAST软件对4型登革病毒的cDNA序列进行序列比对,得到有意义的特异序列;然后用生物学软件Oligo6.0设计特异性高、Tm值相近、长度均一的Oligo探针。结果获得48条70-mer Oligo探针,用于打印成DNA芯片,进行登革病毒的检测。结论利用BLAST系统和生物学软件Oligo6.0设计登革病毒诊断芯片的探针,是一种简便可行、有效的方法。
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| 关 键 词： | 登革病毒/生物信息 生物芯片 Oligo探针 BLAST软件 |
| 文章编号： | 1000-2588(2003)09-0905-03 |
| 修稿时间： | 2003年2月28日 |
Bioinformatic analysis of dengue virus cDNAs and design of oligonucleotide probes for microarray detection of the virus |
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| XIAO Wei-wei ,MA Wen-li ,MA Xiao-dong ,MAO Xiang-ming ,ZHENG Wen-ling Institute of Molecular Biology,First Military Medical University,Guangzhou ,China.Bioinformatic analysis of dengue virus cDNAs and design of oligonucleotide probes for microarray detection of the virus[J].Journal of Southern Medical University,2003,23(9):905-907. |
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| Authors: | XIAO Wei-wei MA Wen-li MA Xiao-dong MAO Xiang-ming ZHENG Wen-ling Institute of Molecular Biology First Military Medical University Guangzhou China |
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| Institution: | XIAO Wei-wei 1,MA Wen-li 1,MA Xiao-dong 1,MAO Xiang-ming 1,ZHENG Wen-ling 21 Institute of Molecular Biology,First Military Medical University,Guangzhou 510515,China, 2 Institute of Molecular Oncology,Guangzhou General Hospital of Guangzhou Command,Guangzhou 510010,China |
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| Abstract: | Objective To design oligonucleotide probes for microarray detection of dengue virus. Methods By analyzing the cDNAs of dengue viruses of 4 different serotypes with BLAST program, a group of specific sequences for the candidate probes was acquired. Oligo6.0 software was applied to analyze the candidates to select the probes with high specificity, identi-cal length and similar melting temperature (Tm). Result Altogether 48 oligonucleotide probes were designed, and deposited on oligonucleotide chips as the microarray for dengue virus detection. Conclusion BLAST program and Oligo6.0 software are simple and effective means for designing the oligonucleotide probes. |
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| Keywords: | dengue virus/bioinformation biochip oligonucleotide probe Basic Local Alignment Search Tool (BLAST) |
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