| 肉苁蓉总苷体外清除自由基及对OH·引发的DNA损伤的保护作用 |
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| 引用本文： | 王晓雯 蒋晓燕 邬利娅.伊明,王雪飞 阿不都热衣木.玉苏甫.肉苁蓉总苷体外清除自由基及对OH·引发的DNA损伤的保护作用[J].中国药学杂志,2001,36(1):29-32. |
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| 作者姓名： | 王晓雯 蒋晓燕 邬利娅.伊明 王雪飞 阿不都热衣木.玉苏甫 |
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| 作者单位： | 王晓雯（新疆医科大学药理教研室，新疆
乌鲁木齐，830054）;蒋晓燕（新疆医科大学药理教研室，新疆 乌鲁木齐，830054）;邬利娅.伊明（新疆医科大学药理教研室，新疆
乌鲁木齐，830054）;王雪飞（新疆医科大学药理教研室，新疆 乌鲁木齐，830054）;阿不都热衣木.玉苏甫（新疆维吾尔医研究
所自由基室，新疆 乌鲁木齐 830001） |
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| 基金项目： | 新疆维吾尔自治区科委资助课题(96814) |
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| 摘 要： | 目的研究肉苁蓉总苷体外清除自由基作用及对OH·引起的DNA损伤的保护作用。方法采用现代生物化学发光技术，检测肉苁蓉总苷对O2，OH·，H2O2等自由基的清除作用。并通过Cu2-Phen-H2O2-VitC-DNA发光体系研究肉苁蓉总苷对OH．引起的DNA氧化损伤的保护作用。结果肉苁蓉总苷对O2，OH·，H2O2活性氧自由基均有清除作用。其50%抑制浓度( IC50)分别为0.0731，7.031，0.098，0.1254mg·mL-1。对DNA化学发光的IC50为0.4211ug·mL-1。结论实验结果表明，肉苁蓉总苷能有效地清除各种活性氧自由基，能保护OH·引发的DNA氧化损伤。
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| 关 键 词： | 肉苁蓉总苷 自由基 DNA损伤 |
| 文章编号： | 1001-2494(2001)01-0029-04 |
| 收稿时间： | 2000-05-09; |
| 修稿时间： | 2000年5月9日 |
Scavenging effects of
glycosides of Cistanche on free radicals and its Protection against OH |
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| WANG Xiao-wen.Scavenging effects of
glycosides of Cistanche on free radicals and its Protection against OH[J].Chinese Pharmaceutical Journal,2001,36(1):29-32. |
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| Authors: | WANG Xiao-wen |
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| Institution: | 1.Department of Pharmacology of Xin Jiang Medical College,Urumuqi,830054,China;2.Free Radica Research sector of Institr.ue of TradiLional Uighur Medicine,Urumuqi,830001,China |
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| Abstract: | OBJECTIVE The extrasomatic free radical scavenging effects of glycosides of Crstanche (GCs) and its protective effects against OH·-induced DNA damage were explored. METHODS The free radical scavenging effects of GCs were measured by Chemiluminescence methods and the protective effects against OH·-induced DNA damage were measured with Cu2+-phen-H2O2-VitC-DNA Chemiluminescence system.RESULTS GCs inhibited the O2，OH·，H2O2 and their 50% of inhibitory concentration(IC50) were 0.0731,7.031,0.098,0.1254mg.·mL-1, respectively. IC50 for DNA Chemiluminescence intensity of GCs was0.4211ug·mL-1.
CONCLUSIONS GCs could scavenge free radicals effectively and has protection against OH·-induced DNA damage. |
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| Keywords: | glycosides of Crstanche Free radical |
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