| 利用pcDNA3．1／TOPOC TA克隆构建ANNEXIN A2基因的真核表达载体 |
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| 引用本文： | 黄昀 金焰 于旸 刘芳莉 傅松滨. 利用pcDNA3．1／TOPOC TA克隆构建ANNEXIN A2基因的真核表达载体[J]. 中国地方病学杂志, 2006, 25(3): 323-325 |
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| 作者姓名： | 黄昀 金焰 于旸 刘芳莉 傅松滨 |
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| 作者单位： | 哈尔滨医科大学医学遗传学教研室,150081 |
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| 基金项目： | 国家自然科学基金资助项目（30471949）;高等学校优秀青年教师教学科研奖励计划（2002）;黑龙江省科学技术攻关项目（GB03C601-1）;黑龙江省教育厅资助项目（10513028）;黑龙江省卫生厅资助项目（2003-104） |
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| 摘 要： | 目的克隆人类ANNEXIN A2基因，构建其正义真核表达质粒。方法采用逆转录聚合酶链式反应技术，从人类正常肺组织中扩增出人类ANNEXIN A2基因的完整编码区全长序列。通过DNA重组技术将该基因重组于pcDNA3．1／TOPO TA真核表达载体上，构建出人类ANNEXIN A2基因正义表达质粒。通过DNA测序方法对重组表达质粒进行鉴定。结果通过测序分析证实，构建的重组表达质粒目的基因片段为人类ANNEXIN A2基因的完整编码区1032bp。结论构建真核表达载体的方法快速简便．适于进一步研究基因的细胞生物学作用。
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| 关 键 词： | ANNEXIN A2基因 DNA 重组 遗传载体 基因表达 |
| 收稿时间： | 2005-05-08 |
| 修稿时间： | 2005-05-08 |
Comtruction of ANNEXIN A2 gene eukaryotic expressive vector by pcDNA3.1/TOPO TA plasmids |
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| HUANG Yun, JIN Yan, YU Yang, LIU Fang-li, FU Song-bin.. Comtruction of ANNEXIN A2 gene eukaryotic expressive vector by pcDNA3.1/TOPO TA plasmids[J]. Chinese Jouranl of Endemiology, 2006, 25(3): 323-325 |
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| Authors: | HUANG Yun JIN Yan YU Yang LIU Fang-li FU Song-bin. |
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| Abstract: | Objective To clone and construct A NNEXIN A 2 gene cloning vector. Methods Human ANNEXIN A2 complete coding region was amplified by RT-PCR method from human normal lung tissues, and was reconstructed into the pcDNA3.1/TOPO TA eukaryotic expressive vector, The reconstructed DNA was identified by DNA sequencing. Results The cloned DNA was confirmed to be human ANNEXIN A2 CDS by DNA sequencing. Conclusions Recombinant DNA with cloning vector, an efficient method, has laid the foundation of further biological study. |
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| Keywords: | Genes,A NNEXIN A 2 DNA, recombinant Genetic vectors Gene expression |
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