| 角质细胞生长因子2的优化表达及纯化研究 |
| |
| 引用本文： | 林来兴妹,孟晓静,王小宁,吉程程,罗林,亦伟,龙滨,张钧.角质细胞生长因子2的优化表达及纯化研究[J].南方医科大学学报,2001(Z1). |
| |
| 作者姓名： | 林来兴妹 孟晓静 王小宁 吉程程 罗林 亦伟 龙滨 张钧 |
| |
| 作者单位： | 第一军医大学分子免疫学研究所，第一军医大学分子免疫学研究所，第一军医大学学员一旅四队，第一军医大学学员一旅四队，第一军医大学学员一旅四队，第一军医大学学员一旅四队，第一军医大学学员一旅四队，第一军医大学分子免疫学研究所 广东 广州 510515，广东 广州 510515，广东 |
| |
| 摘 要： | 目的优化工程菌的表达,比较肝素亲和层析、离子交换层析两种方法的角质细胞生长因子2（keratinocyte growthfactor,KGF-2）的纯化产量及纯度,以获取高效的提纯工艺。方法工程菌分别进行生长密度和诱导时间的实验,并将细茵裂解液上两种层析柱进行分步洗脱,收集目标蛋白,进行电泳分析。结果获得高表达KGF-2工程菌,表达率为29％;用两种层析方法获得电泳纯KGF-2。结论 离子交换层析在纯化过程中具有一定优势。
|
| 关 键 词： | 角质细胞生长因子2 纯化 |
Optimized expression and purification of keratinocyte growth factor-2 |
| |
| LINLAI Xing-mei,MENG Xiao-jing,JI Cheng-cheng,LUO Lin,YI Wei,LONG Bin,ZHANG Jun,WANG Xiao-ningInstitute of Molecular Immunology, Fourth Corps,First Cadet Brigade,First Military Medical University,Guangzhou ,China.Optimized expression and purification of keratinocyte growth factor-2[J].Journal of Southern Medical University,2001(Z1). |
| |
| Authors: | LINLAI Xing-mei MENG Xiao-jing JI Cheng-cheng LUO Lin YI Wei LONG Bin ZHANG Jun WANG Xiao-ningInstitute of Molecular Immunology Fourth Corps First Cadet Brigade First Military Medical University Guangzhou China |
| |
| Institution: | LINLAI Xing-mei,MENG Xiao-jing,JI Cheng-cheng,LUO Lin,YI Wei,LONG Bin,ZHANG Jun,WANG Xiao-ningInstitute of Molecular Immunology, Fourth Corps,First Cadet Brigade,First Military Medical University,Guangzhou 510515,China |
| |
| Abstract: | Objective To optimize the expression of keratinocyte growth factor-2 (KGF-2) and to evaluate heparin affinity chromatography and ion-exchange chromatography in view of KGF-2 output and purity. Methods Expression of KGF-2 was optimized on growth density and induction time. The bacteria lytic fluid underwent the 2 chromatographic treatment and eluted in serial concentration. The solution was subsequently collected and analyzed by SDS-PAGE. Results The engineering bacteria that expressed KGF-2 at a rate as high as 29% was obtained. Conclusion Ion-exchange chromatography proves to be better than heparin affinity chromatography in purifying of KGF-2. |
| |
| Keywords: | keratinocyte growth factor-2 purification |
| 本文献已被 CNKI 等数据库收录！ |
|
