载玻片代替MACRO精子计数板可行性分析

目的探讨计算机辅助精液分析（CASA）中以普通载玻片代替MACRO精子计数板的可行性。方法对前来就诊的患者按要求留取精液，液化后分别取适量标本放在MACRO精子计数板和普通载玻片上采用WLJY-9000伟力彩色精子质量检测系统分别测得其密度、活率及活力等参数。结果在少精组（精子密度每毫升小于20×10^6）、弱精组（精子活率小于50％）、少弱精组（精子密度每毫升小于20×10^6，且精子活率小于50％）和正常组（精子密度每毫升大于20×10^6，且精子活率大于50％）间分别用MACRO精子计数板和普通载玻片测得其密度、活率及活力各参数间差异无统计学意义。结论CASA中以普通载玻片代替MACRO精子计数板操作简便、结果可靠、经济实用。

Feasibility analysis of ordinary glass slide substituting for the MACRO sperm counting plate during computer-aided semen analysis

Objective To investigate the feasibility analysis of ordinary glass slide substituting for the MACRO sperm counting plate during computer-aided semen analysis. Methods The liquefactive semen samples were smeared respectively on the MACRO sperm counting plate and ordinary glass slide, and measured for spermatozoa density, motility rate and vital force by applying WLJY-9000 colour semen analysis system. According to detected results, the semen samples were divided into few spermatozoa group （spermatozoa density was below 20 × 10^6/mL）, weak spermatozoa group （spermatozoa motility rate was below 50%）, few and weak spermatozoa group （spermatozoa density was below 20 × 10^6/mL and motility rate was below 50%）, normal spermatozoa group （spermatozoa density and activity were both normal）. Results The differences in all the three detected parameters （spermatozoa density, motility rate and vital force） weren＇t statistical significant in each group between MACRO sperm counting plate method and ordinary glass slide method. Conclusion The ordinary glass slide method substitution for MACRO sperm counting plate way is simple, reliable and practical during computer-aided semen analysis （CASA）.