| 色氨酸对蟾蜍卵母细胞膜电位的影响 |
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| 引用本文： | 王凯,付纯芳,于翔辉,王羽峰,杨殿生,张桂芳.色氨酸对蟾蜍卵母细胞膜电位的影响[J].黑龙江医药科学,1997(3). |
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| 作者姓名： | 王凯 付纯芳 于翔辉 王羽峰 杨殿生 张桂芳 |
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| 作者单位： | 佳木斯医学院生理教研室，佳木斯医学院生理教研室，佳木斯医学院生理教研室，佳木斯医学院生理教研室，佳木斯医学院生理教研室，佳木斯医学院生理教研室 |
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| 摘 要： | 本文采用微最极细胞内记录方法观察了色氨酸对蟾蜍卵母细胞膜电位影响和时间效应和剂量效应,色氨酸对裸化卵母细胞膜电位的影响;色氨酸对卵母细胞膜电位起极化作用的离子关系等,其结果是以1mmol/L 色氨酸处理卵母细胞0~-1h 膜电位无明显变化,2h 后膜电位值便随培养时间延长而逐渐升高(超极化),随着色氨酸浓度升高其膜电位也逐渐升高。色氨酸对裸化卵母细胞膜电位有轻度超极化作用,但与对照组无明显差异(p>0.05):色氨酸虽然能使卵细胞膜电位明显超极化,但对细胞膜钠离子转运系数(TNa~ )无明显影响,而且膜电位变化与 TNa~ 变化无相关性。
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| 关 键 词： | 色氨酸 卵母细胞 膜电位 裸母细胞 |
Effect oftryptophan on membrane potential of oocyte in toads |
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| Wang Kai.Effect oftryptophan on membrane potential of oocyte in toads[J].Heilongjiang Medicine and Pharmacy,1997(3). |
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| Authors: | Wang Kai |
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| Abstract: | The tine and dosage-dependent effect of the tryplophan affecting oocyte membrane potential was observed with method of microelectrode in cells.The tryptophan acting on bare oocyte membrane potential was also observed,It is suggested that membarane potential didn't change obviously in short term (0-1 hour)after 1mmol/L trypotophan treating oocyte and membrane potcmtial elevated gradually in 2 hours with culture time prolongating. The tryolophan leads to bare oocyte membrane potential hyperpolarizatig slightly and didn't have statistical significance compared with control group (P/0.05).The trypotophan affects Na~ permeability.It is suggected that the trypotophan causes oocyte membrane potential hyperpolarization distinctiveiy and didn't affect Na~ .The change of membrane potential and Na~-didn't correlate with each other and trypotophan didn't cause membrane potential hyperpolarization. |
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| Keywords: | trypotophan oocyte membrane potential bare oocyte |
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