羊栖菜多糖体外抗病毒作用研究

目的:观察羊栖菜多糖及其分离产物对单纯疱疹病毒1型(HSV-1)和柯萨奇病毒(CVB₃)所引起细胞病变(CPE)的抑制作用及其机理。方法:采用MTT比色法,测定羊栖菜多糖及其分离产物对Vero细胞的细胞毒性,并在对细胞的最大无毒浓度(MNCC)范围内,采用体外细胞培养法研究羊栖菜多糖样品对上述两种病毒的抑制作用。在此基础上采用分组实验初步探讨了多糖样品的抗病毒机理。结果与结论:①羊栖菜多糖样品对Vero细胞的毒性较小,CC₅₀均大于5000mg/L。②除ALG外,其它样品对HSV-1均有明显的抗病毒作用,且随着纯度的提高,样品抗病毒作用随之增强,其中F₁、F₂和F₄抗病毒效果优于ACV。③羊栖菜多糖样品对CVB3的抗病毒效果均明显优于病毒唑,纯化后的样品F₁、F₂和F₄抗CVB₃作用显著。④从分组实验中可知,羊栖菜多糖样品不仅具有直接杀灭病毒作用,而且还可进入细胞或吸附在细胞表面,从而达到抑制或杀伤病毒的效果。

Antivirus effects of polysaccharides from Sargassum fusiforme in vitro

AIM: To assay antiviral effects of the polysaccharide and separated products from Sargassum fusiforme on herpes simplex virus type 1 (HSV-1) and coxsackie virus (CVB₃) and their antiviral mechanism. METHODS: The antiviral effects of all extracts from Sargassum fusiforme on HSV-1 and CVB₃ and their cytotoxicity on Vero cells were studied by CPE and MTT methods. Antiviral mechanism of two extracts was also investigated by four different ways. RESULTS and CONCLUSION: ① The cytotoxicity of extracts on Vero cells was undetectable (CC₅₀＞5 000 mg/L). ② All extracts except algin (ALG) had an obviously HSV-1 inhibitory effect. Antiviral activities of them were increasingly strengthened with their purities. Antiviral activities of extracts from flucoidan 1, F₁, F₂ and F₄ were better than acyclovir (ACV). ③ Antiviral activities of all extracts on CVB₃ were better than ribavirin injection. F₁, F₂ and F₄ had remarkable anti-CVB₃ effects. ④ The experiment showed that Sargassum fusiforme polysaccharides not only kill the above viruses directly but also restrain them via getting into cells or absorbing on cells.