玉叶清火胶囊中玉叶金花鉴别方法的研究

目的:考查玉叶清火胶囊中玉叶金花的鉴别方法。方法:通过改变提取方法、洗脱剂、展开剂选择薄层色谱法(TLC)中的最佳实验条件。结果:经大孔树脂处理,20%～30%之间的乙醇洗脱,以三氯甲烷-甲醇-水(13:6:2)10℃以下放置分层的下层溶液为展开剂得到的薄层色谱图中,玉叶金花与对照药材色谱在相应的位置上,显相同颜色的斑点,且斑点清晰,分离效果好,阴性对照无干扰。结论:实验证明此方法简便、快捷,能准确鉴别出玉叶清火胶囊中的玉叶金花。

The Methods of Identifying Mussaenda Pubescens in Yuyeqinghuo Capsules

Objectives: To examine the methods of how to identify Mussaenda pubescens in Yuyeqinghuo capsules.Methods: By changing the techniques of extraction,eluting agent and the optimum experiment conditions of TLC.Results: The thin-layer chromatography(TLC) got by the means of treated by macroporous resin first,then using 20%~30% alcohol to elute and the lower parts of the layered solution of chloroform-methanol-water(13:6:2) under 10℃ as developing solvent shows that the mussaenda pubescens and control drug display spots of the same color.Furthermore the spots are quite clear and the separation effects are good;there is no interference for the negative control group.Conclusion: Experiments show that this method is simple,fast,Can accurately identified the golden jade leaf jade leaf lowering capsule