| 人HOXA1 3'-非翻译区荧光素酶报告基因载体的构建及鉴定 |
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| 引用本文： | 肖法嫚,李余发,陈珍珠,曾香萍,郭琳琅.人HOXA1 3'-非翻译区荧光素酶报告基因载体的构建及鉴定[J].数理医药学杂志,2012,25(4):407-409. |
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| 作者姓名： | 肖法嫚 李余发 陈珍珠 曾香萍 郭琳琅 |
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| 作者单位： | 1. 南方医科大学附属珠江医院肿瘤科,广州,510282
2. 南方医科大学附属珠江医院病理科 |
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| 基金项目： | 国家自然科学基金项目资助，广东省自然基金项目资助 |
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| 摘 要： | 目的:针对人同源异型盒A1(HOXA1)基因的3'端非翻译区(3'-untranslated region,3'-UTR)构建HOXA1的荧光素酶报告基因载体,对其克隆进行鉴定并挑选出正确的克隆,为后续功能研究提供基础。方法:PCR扩增包含HOXA1 3'-UTR的DNA片段,克隆至荧光素酶载体psiCHECK-2,构建HOXA1 3'-UTR荧光素酶报告基因载体,经Not I和Xho I双酶切后测序进行鉴定。结果:克隆获得的DNA片段大小及序列与GenBank报道的一致,且插入方向正确。结论:成功构建了含hURAT1基因3'UTR区的荧光素酶报告载体,可用于后续功能研究。
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| 关 键 词： | 同源异型盒A1基因 荧光素酶报告基因 3'-非翻译区 |
Construction of the Luciferase Reporter Gene Vector Containing HOXA1 3'UTR |
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| Institution: | Xiao Faman,et al(Department of Oncology,The Affiliated Zhujiang Hospital of Southern Medical University,Guangzhou 510282) |
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| Abstract: | Objective: To construct the luciferase reporter gene vector containing 3’-untranslated region(3’-UTR) of human HOXA1,which will provide basis for subsequnent fuction research.Methods: The 3’-UTR of HOXA1 gene were amplified with the human genome DNA by PCR.The segment was cloned into the luciferase vector psiCHECK-2.The recombinants were restriction enzyme digested by Not I and Xho I and sequenced.Results: The sequenced segment obtained in the recombinants was identical to that reported by GenBank and the segment inserted in the correct direction.Conclusion: The luciferase expression recombinant containing 3’-UTR of HOXA1 was successfully constructed,which can be used for subsequent function research. |
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| Keywords: | HOXA1 luciferase reporter gene 3-untranslated region |
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