| 双花草珊瑚含片中绿原酸的含量测定 |
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| 引用本文： | 唐德智. 双花草珊瑚含片中绿原酸的含量测定[J]. 安徽医药, 2009, 13(11): 1357-1358 |
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| 作者姓名： | 唐德智 |
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| 作者单位： | 广西南宁食品药品检验所,广西,南宁,530001 |
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| 摘 要： | 目的建立双花草珊瑚含片中绿原酸的含量测定方法。方法采用Shim-packVP—ODSC18反相色谱柱（250mm×4．6mm，5μm），以乙腈-0．2％磷酸（12：88）为流动相；流速为1．0ml·min^-1；检测波长327nm；柱温：30℃。结果绿原酸在0．101—0．708μg范围内线性关系良好（r=0．9999，n=7），平均加样回收率为99．3％，RSD为1．2％（n=6）。结论本方法操作简便，结果准确、可靠，可用于双花草珊瑚含片中绿原酸的含量测定。
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| 关 键 词： | 双花草珊瑚含片 绿原酸 反相高效液相色谱法 |
Determination of chlorogenic acid in shuanghua caoshanhu hanpian |
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| TANG De-zhi. Determination of chlorogenic acid in shuanghua caoshanhu hanpian[J]. Anhui Medical and Pharmaceutical Journal, 2009, 13(11): 1357-1358 |
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| Authors: | TANG De-zhi |
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| Affiliation: | TANG De-zhi(Nanning Institute for Food , Drug Control,Nanning Guangxi 530001,China) |
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| Abstract: | Aim To establish a method for the determination of chlorogenie acid in shuanghua caoshanhu hanpian by RP - HPLC. Methods The chromatographic column of Shim - pack VP - ODS C,s （ 250 mmx 4.6 mm, 5 μm ） was used, the mobile phase consisting of acetonitrile -0. 2% phosphoric acid （12：88）. The flow rate was 1.0 ml · min^-1. The detection wavelength was set at 327 nm and the column temperature was maintained at 30℃. Results The linear range was 0.101 - 0. 708 μg （ r = 0. 999 9, n = 6）, The average recovery was 99.3 %, wiith the RSD of 1.2% （ n = 6）. Conclusion The method is convenient, accurate and suitable for the determination of chlorogenic acid in shuanghua caoshanhu hanpian. |
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| Keywords: | shuanghua caoshanhu hanpian chlorogenic acid RP-HPLC |
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