Ezrin、FAK及E-cadherin在大肠癌组织中的表达及其临床意义

目的:探讨大肠癌组织中埃兹蛋白(Ezrin),黏着斑激酶(FAK)及上皮细胞钙黏蛋白(E-cadherin)的表达及其与肿瘤侵袭和转移的关系.方法:大肠癌组织标本50例,其中高分化腺癌13例,中低分化腺癌37例;无淋巴结转移30例,淋巴结转移20例.采用免疫组织化学方法检测50例大肠癌组织中Ezrin,FAK及E-cadherin的蛋白表达,并运用统计学方法分析Ezrin,FAK及E-cadherin的表达与大肠癌各种临床病理特征的关系及三者的相关性.结果:Ezrin在中低分化、伴有淋巴结转移及Dukes C D期大肠癌的阳性表达率显著高于高分化、无淋巴结转移及Dukes A B期大肠癌(83.78% vs 46.15%,P<0.01;95.00%vs 60.00%,P<0.01;95.00% vs 60.00%,P<0.01),而E-cadherin在以上组织中的表达正好相反(24.32% vs 69.23%,P<0.01;10.00% vs 53.33%,P<0.01;10.00% vs 53.33%,P<0.01),其均与患者性别及年龄大小均无关(P>0.05).FAK在Dukes C D期、伴有淋巴结转移大肠癌组织中的阳性表达显著高于Dukes A B、无淋巴转移组织(100.00% vs 63.33%,P<0.01;100.00% vs 63-33%,P<0.01),而与肿瘤的分化程度、患者性别及年龄大小均无关(P>0.05).经Spearman相关分析,Ezrin与FAK在大肠癌组织中的表达呈正相关(r=0.346,P<0.05),E-cadherin与Ezrin、FAK在大肠癌组织中的表达均呈明显负相关(r=-0.410,P<0.01;r=-0.406,P<0.01).结论:Ezrin,FAK及E-cadherin在大肠癌组织中的异常表达与肿瘤组织的浸润和转移密切相关,联合检测可以作为一组有效的大肠癌肿瘤标记和预后指标.

Expression of ezrin, E-cadherin and focal adhesion kinase in colorectal carcinoma and their clinical significances

AIM:To investigate the expression of ezrin,focal adhesion kinase (FAK) and E-cadherin (E-cad) as well as their correlations with the invasion and metastasis of colorectal carcinoma. METHODS:Immunohistochemistry was used to detect the expression of ezrin,FAK and E-cad in 50 cases of colorectal carcinoma (including 13 well-differentiated adencarcinomas,37 moder- ately- or poorly-differentiated adenocarcinomas; 30 cases without lymph node metastasis and 20 with lymph node metastasis).The correlations among Ezrin,FAK and E-cadherin expression as well as the clinical and pathological characteris- tics were analyzed by statistical method. RESULTS:The expression of ezrin was sig- nificantly higher in colorectal carcinomas with moderate or poor differention,lymph node me- tastasis and Dukes C D stages than that in the ones with high differention,non-metastasis,and Dukes A B stages (83.78% vs 46.15%,P<0.01; 95.00% vs 60.00%,P<0.01;95.00% vs 60.00%,P<0.01),but E-cad expression was in the contrary situation (24.32% vs 69.23%,P<0.01;10.00% vs 53.33%,P<0.01;10.00% vs 53.33%,P<0.01).The expression of ezrin and E-cad had no marked correlations with the age and sex of patients (P>0.05).FAK expression was markedly higher in colorectal carcinomas with Dukes C D stages and lymph node metastasis than that in the ones with Dukes A B stages and without metastasis (100.00% vs 63.33%,P<0.01;100.00% vs 63.33%, P<0.01),but it had no significant correlation with the differetion degree of tumors,the sex and ages of patients (P>0.05).Spearman analy- sis showed that there existed positive correlation between Ezrin and FAK expression (r=0.346,P<0.05),and negative correlation between E-cad- herin and Ezrin (r=-0.410,P<0.01) as well as E-cadherin and FAK expression (r=-0.406,P<0.01). CONCLUSION:The abnormal expression of ezrin,FAK and E-cad are closely correlated with the infiltration and metastasis of colorectal carci- noma,and the combined detection can be used to judge the prognosis of patients with colorectal carcinoma.